Standard Curve (Sample)
(Human CXCL13 PicoKine ELISA Kit standard curve)
Human CXCL13/BLC/BCA1 ELISA Kit | CXCL13 elisa kit
Human CXCL13/BLC/BCA1 PicoKine Quick ELISA Kit
Gene Names
CXCL13; BLC; BCA1; ANGIE; BCA-1; BLR1L; ANGIE2; SCYB13
Reactivity
Human
Applications
ELISA
Synonyms
CXCL13/BLC/BCA1; N/A; Human CXCL13/BLC/BCA1 PicoKine Quick ELISA Kit; CXCL13 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human CXCL13
Assay Type
Sandwich
Samples
Cell culture supernatants, serum, plasma (heparin, EDTA) and saliva
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<1 pg/ml
Applicable Applications for CXCL13 elisa kit
ELISA
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Standard Curve (Sample)
(Human CXCL13 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human CXCL13 PicoKine ELISA Kit standard curve)
Related Product Information for CXCL13 elisa kit
Background: C-X-C motif chemokine 13(CXCL13), also known as B lymphocyte chemoattractant(BLC), is a protein that in humans is encoded by theCXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family, and it was located on sequence maps to 4q21, near the genes encoding most CXC chemokines. In T-lymphocytes, CXCL13 expression is thought to reflect a germinal center origin of the T-cell. Hence, expression of CXCL13 in T-cell lymphomas, such as Angioimmunoblastic T-cell Lymphoma, is thought to reflect a germinal center origin of the neoplastic T-cells
Principle of the Assay: The Quick Picokine™ Human CXCL13 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CXCL13 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit includes Human CXCL13 protein as standards. To measure Human CXCL13, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Human CXCL13 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CXCL13 in the sample.
Principle of the Assay: The Quick Picokine™ Human CXCL13 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CXCL13 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit includes Human CXCL13 protein as standards. To measure Human CXCL13, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Human CXCL13 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CXCL13 in the sample.
Product Categories/Family for CXCL13 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
12,664 Da
NCBI Official Full Name
C-X-C motif chemokine 13
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 13
NCBI Official Symbol
CXCL13
NCBI Official Synonym Symbols
BLC; BCA1; ANGIE; BCA-1; BLR1L; ANGIE2; SCYB13
NCBI Protein Information
C-X-C motif chemokine 13; B-cell chemoattractant; B-cell-attracting chemokine 1; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); B-lymphocyte chemoattractant; CXC chemokine BLC; b cell-attracting chemokine 1; b lymphocyte chemoattracta
UniProt Protein Name
C-X-C motif chemokine 13
UniProt Gene Name
CXCL13
UniProt Synonym Gene Names
BCA1; BLC; SCYB13; BCA-1
UniProt Entry Name
CXL13_HUMAN
Similar Products
Product Notes
The Human CXCL13 cxcl13 (Catalog #AAA126811) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA126811 ELISA Kit recognizes Human CXCL13. AAA Biotech's CXCL13/BLC/BCA1 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the CXCL13 cxcl13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CXCL13/BLC/BCA1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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