Standard Curve (Sample)
Human Cytochrome P450 17A1 (CYP17A1) ELISA Kit | CYP17A1 elisa kit
Human Cytochrome P450 17A1 (CYP17A1) ELISA Kit
Gene Names
CYP17A1; CPT7; CYP17; S17AH; P450C17
Reactivity
Human
Synonyms
Cytochrome P450 17A1 (CYP17A1); N/A; Human Cytochrome P450 17A1 (CYP17A1) ELISA Kit; S17aH; CPT7; P450C17; 17-Alpha-Hydroxylase; 17-alpha-hydroxyprogesterone aldolase/17; 20-Lyase; Steroid 17-alpha-monooxygenase; Cytochrome P450; Family 17; Subfamily A; Polypeptide 1; CYP17A1/Cytochrome P450 17A1; CYP17A1 elisa kit
Reactivity
Human
Specificity
Reactive with Human CYP17A1/Cytochrome P450 17A1
This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 17A1 (CYP17A1). No significant cross-reactivity or interference between Cytochrome P450 17A1 (CYP17A1) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 17A1 (CYP17A1). No significant cross-reactivity or interference between Cytochrome P450 17A1 (CYP17A1) and analogues was observed.
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
0.054ng/mL
Detection Character
Regular detection
Assay Time
3h
Sample Size
100ul
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 17A1 (CYP17A1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 17A1 (CYP17A1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%, Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 17A1 (CYP17A1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%, Inter-Assay: CV<12%
Assay Procedure Summary
1. Prepare all reagents, samples and standards
2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37 degree C
8. Add 50uL Stop Solution. Read at 450nm immediately.
2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37 degree C
8. Add 50uL Stop Solution. Read at 450nm immediately.
Test Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 17A1 (CYP17A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 17A1 (CYP17A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 17A1 (CYP17A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 17A1 (CYP17A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Preparation and Storage
Store at 4 degree C for 1 month/-20 degree C for long-term (within shelf life)
Shelf Life: 8 months
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Shelf Life: 8 months
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Standard Curve (Sample)
Product Categories/Family for CYP17A1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,371 Da
NCBI Official Full Name
steroid 17-alpha-hydroxylase/17,20 lyase
NCBI Official Synonym Full Names
cytochrome P450, family 17, subfamily A, polypeptide 1
NCBI Official Symbol
CYP17A1
NCBI Official Synonym Symbols
CPT7; CYP17; S17AH; P450C17
NCBI Protein Information
steroid 17-alpha-hydroxylase/17,20 lyase; CYPXVII; cytochrome P450c17; cytochrome P450-C17; cytochrome P450 17A1; cytochrome p450 XVIIA1; steroid 17-alpha-monooxygenase; 17-alpha-hydroxyprogesterone aldolase; cytochrome P450, subfamily XVII (steroid 17-al
UniProt Protein Name
Steroid 17-alpha-hydroxylase/17,20 lyase
UniProt Gene Name
CYP17A1
UniProt Synonym Gene Names
CYP17; S17AH; Cytochrome P450c17
UniProt Entry Name
CP17A_HUMAN
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Product Notes
The Human CYP17A1 cyp17a1 (Catalog #AAA189772) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA189772 ELISA Kit recognizes Human CYP17A1. It is sometimes possible for the material contained within the vial of "Cytochrome P450 17A1 (CYP17A1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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