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IHC (Immunohistochemistry)
(AAA321877 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human, Mouse Cytochrome P450 17A1 Polyclonal Antibody | anti-CYP17A1 antibody
Cytochrome P450 17A1 Antibody
Gene Names
CYP17A1; CPT7; CYP17; S17AH; P450C17
Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Cytochrome P450 17A1, Antibody; Cytochrome P450 17A1 Antibody; 20 lyase; CP17A_HUMAN; CPT7; CYP17; CYP17A1; CYPXVII; Cytochrome P450 17A1; Cytochrome P450 family 17; Cytochrome P450 family 17 subfamily A polypeptide 1; Cytochrome p450 subfamily XVII (steroid 17 alpha hydroxylase) adrenal hyperplasia; Cytochrome p450 XVIIA1; Cytochrome P450-C17; Cytochrome P450c17; OTTHUMP00000020382; P450 C17; P450c17; S17AH; Steroid 17 alpha hydroxylase/17, 20 lyase; Steroid 17 alpha monooxygenase; Steroid 17-alpha-hydroxylase/17; Steroid 17-alpha-monooxygenase; anti-CYP17A1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Cytochrome P450 17A1 antibody detects endogenous levels of total Cytochrome P450 17A1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
508
Applicable Applications for anti-CYP17A1 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human Cytochrome P450 17A1
Subcellular Location
Membrane.
Predicted Cross Reactivity
Rabbit, Dog
Similarity
Rabbit (80%), Dog (90%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohistochemistry)
(AAA321877 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA321877 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA321877 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA321877 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemisry)
(AAA321877 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemisry)
(AAA321877 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence)
(AAA321877 staining LOVO cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
IF (Immunofluorescence)
(AAA321877 staining LOVO cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
WB (Western Blot)
(Western blot analysis of Cytochrome P450 17A1 expression in mouse tissue.)
WB (Western Blot)
(Western blot analysis of Cytochrome P450 17A1 expression in mouse tissue.)
Related Product Information for anti-CYP17A1 antibody
Description: Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17, 20-lyase reaction. Involved in sexual development during fetal life and at puberty.
Post-translational Modifications: Phosphorylation is necessary for 17, 20-lyase, but not for 17-alpha-hydroxylase activity.
Similarity: Belongs to the cytochrome P450 family.
Post-translational Modifications: Phosphorylation is necessary for 17, 20-lyase, but not for 17-alpha-hydroxylase activity.
Similarity: Belongs to the cytochrome P450 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 57 kDa
Predicted: 58 kDa
Predicted: 58 kDa
NCBI Official Full Name
steroid 17-alpha-hydroxylase/17,20 lyase
NCBI Official Synonym Full Names
cytochrome P450 family 17 subfamily A member 1
NCBI Official Symbol
CYP17A1
NCBI Official Synonym Symbols
CPT7; CYP17; S17AH; P450C17
NCBI Protein Information
steroid 17-alpha-hydroxylase/17,20 lyase
UniProt Protein Name
Steroid 17-alpha-hydroxylase/17,20 lyase
UniProt Gene Name
CYP17A1
UniProt Synonym Gene Names
CYP17; S17AH; Cytochrome P450c17
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Product Notes
The CYP17A1 cyp17a1 (Catalog #AAA321877) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cytochrome P450 17A1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome P450 17A1 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CYP17A1 cyp17a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome P450 17A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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