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product-image-AAA21474_SC.jpg Standard Curve (Sample)

Human DAO ELISA Kit | DAO elisa kit

Human DAO (Diamine Oxidase) ELISA Kit

Gene Names
AOC1; ABP; DAO; KAO; ABP1; DAO1
Reactivity
Human
Synonyms
DAO; N/A; Human DAO (Diamine Oxidase) ELISA Kit; DAO elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This kit recognizes Human DAO in samples. No significant cross-reactivity or interference between Human DAO and analogues was observed
Sequence Length
751
Assay Type
Quantitative Sandwich
Samples
Serum, plasma and other biological fluids
Detection Range
3.13-200 ng/mL
Sensitivity
1.88 ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human DAO were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human DAO were tested on 3 different plates, 20 replicates in each plate, respectively.
Preparation and Storage
Store at 4 degree C.

Standard Curve (Sample)

product-image-AAA21474_SC.jpg Standard Curve (Sample)
Related Product Information for DAO elisa kit
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DAO. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DAO and AvidinHorseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DAO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human DAO. You can calculate the concentration of Human DAO in the samples by comparing the OD of the samples to the standard curve.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human DAO concentrations in serum, plasma and other biological fluids.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
26
UniProt Accession #
Molecular Weight
87,239 Da
NCBI Official Full Name
Amiloride-sensitive amine oxidase
NCBI Official Synonym Full Names
amine oxidase, copper containing 1
NCBI Official Symbol
AOC1
NCBI Official Synonym Symbols
ABP; DAO; KAO; ABP1; DAO1
NCBI Protein Information
amiloride-sensitive amine oxidase [copper-containing]; amiloride-sensitive amine oxidase [copper-containing]; amiloride binding protein 1 (amine oxidase (copper-containing)); amiloride-binding protein 1; amine oxidase copper domain-containing protein 1; diamine oxidase; histaminase; kidney amine oxidase
UniProt Protein Name
Amiloride-sensitive amine oxidase [copper-containing]
UniProt Gene Name
AOC1
UniProt Synonym Gene Names
ABP1; DAO1; DAO; Diamine oxidase; KAO
UniProt Entry Name
AOC1_HUMAN

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Product Notes

The Human DAO aoc1 (Catalog #AAA21474) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA21474 ELISA Kit recognizes Human DAO. It is sometimes possible for the material contained within the vial of "DAO, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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