Standard Curve (Sample)
Human Dapper Homolog 1 (DACT1) ELISA Kit | DACT1 elisa kit
Human Dapper Homolog 1 (DACT1) ELISA Kit
Gene Names
DACT1; DPR1; TBS2; FRODO; HDPR1; DAPPER; THYEX3; DAPPER1
Reactivity
Human
Synonyms
Dapper Homolog 1 (DACT1); Human Dapper Homolog 1 (DACT1) ELISA Kit; DACT1; DAPPER; DAPPER1; DPR1; FRODO; HDPR1; THYEX3; dapper 1; dapper homolog 1; antagonist of beta-catenin; heptacellular carcinoma novel gene 3; DACT1 elisa kit
Reactivity
Human
Specificity
Human Dapper Homolog 1 (DACT1) ELISA Kit has high sensitivity and excellent specificity for detection of Human DACT1. No significant cross-reactivity or interference between Human DACT1 and analogues was observed.
Sequence Length
836
Assay Type
Sandwich
Samples
Human serum, cell culture supernates and other biological fluids
Detection Range
30 pg/ml - 480 pg/ml
Sensitivity
< 1pg/mL
Intra-assay Precision
Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?9%.
Inter-assay Precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?11%.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for DACT1 elisa kit
Background: Dapper homolog 1 (DACT1) is a disheveled partner in the planar cell polarity pathway. By using genome-wide promoter methylation screening, dapper homolog 1 (DACT1) was found to be frequently methylated in gastric cancer.
Principle of the Assay: Human Dapper Homolog 1 (DACT1) ELISA Kit employs a two-site sandwich ELISA to quantitate DACT1 in samples. An antibody specific for DACT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DACT1 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human DACT1 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of DACT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: Human Dapper Homolog 1 (DACT1) ELISA Kit employs a two-site sandwich ELISA to quantitate DACT1 in samples. An antibody specific for DACT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DACT1 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human DACT1 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of DACT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dapper homolog 1 isoform 1
NCBI Official Synonym Full Names
dishevelled binding antagonist of beta catenin 1
NCBI Official Symbol
DACT1
NCBI Official Synonym Symbols
DPR1; TBS2; FRODO; HDPR1; DAPPER; THYEX3; DAPPER1
NCBI Protein Information
dapper homolog 1
UniProt Protein Name
Dapper homolog 1
UniProt Gene Name
DACT1
UniProt Synonym Gene Names
DPR1; HNG3; hDPR1
UniProt Entry Name
DACT1_HUMAN
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Product Notes
The Human DACT1 dact1 (Catalog #AAA31500) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA31500 ELISA Kit recognizes Human DACT1. It is sometimes possible for the material contained within the vial of "Dapper Homolog 1 (DACT1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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