Human Diablo/SMAC ELISA Kit | DIABLO elisa kit

Human Diablo/SMAC PicoKine ELISA Kit

Cat. Num. AAA45765

• Gene Names: DIABLO; SMAC; DFNA64
• Reactivity: Human
• Synonyms: Diablo/SMAC; N/A; Human Diablo/SMAC PicoKine ELISA Kit; Diablo homolog, mitochondrial; diablo, IAP-binding mitochondrial protein; DFNA64; DIABLO; diablo homolog, mitochondrial; DIABLO-S; direct IAP-binding protein with low pI; mitochondrial Smac protein; second mitochondria-derived activator of caspase; SMAC3; DIABLO elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human Diablo
• Sequence Length: 239
• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: < 10pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Human Diablo/SMAC PicoKine ELISA Kit standard curve)

Related Product Information for DIABLO elisa kit

Principle of the assay: human Diablo ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Diablo has been precoated onto 96-well plates. Standards(E.coli, A56-D239) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Diablo is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Diablo amount of sample captured in plate.
Background: Diablo homolog, mitochondrial, also known as second mitochondria-derived activator of caspases (SMAC), is a protein that in humans is encoded by the DIABLO gene (direct IAP binding protein with low pI). It is mapped to 12q24.31. DIABLO can bind mammalian IAP homolog A (MIHA, or API3) and can also interact with MIHB, MIHC, and OpIAP, the baculoviral IAP. This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Over expression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64.

NCBI and Uniprot Product Information

• NCBI GI #: 18203316
• NCBI GeneID: 56616
• UniProt Accession #: Q9NR28; Q6W3F3; Q96LV0; Q9BT11; Q9HAV6; B2RDQ0
• Molecular Weight: 22,284 Da
• NCBI Official Full Name: Diablo homolog, mitochondrial
• NCBI Official Synonym Full Names: diablo, IAP-binding mitochondrial protein
• NCBI Official Symbol: DIABLO
• NCBI Official Synonym Symbols: SMAC; DFNA64
• NCBI Protein Information: diablo homolog, mitochondrial; direct IAP-binding protein with low pI; second mitochondria-derived activator of caspase
• UniProt Protein Name: Diablo homolog, mitochondrial
• UniProt Gene Name: DIABLO
• UniProt Synonym Gene Names: SMAC; Smac
• UniProt Entry Name: DBLOH_HUMAN