IHC (Immunohistochemisry)
(Fig: 3 Formalin-fixed, paraffin-embedded human colon stained for Smac/DIABLO using at 1: 2000. A, normal colon. B and C, colon carcinoma. Only trace Smac/DIABLO immunoreactivity is seen in normal colonic epithelium (A), which is seen in the apical, foveolar cells. In contrast, strong immunoreactivity is observed in nuclear (B) and cytoplasmic (C) locations in the colon carcinoma specimen. Hematoxylin-eosin counterstain.)
Rabbit SMAC/Diablo Polyclonal Antibody | anti-DIABLO antibody
Polyclonal Antibody to SMAC/Diablo
Gene Names
DIABLO; SMAC; DFNA64
Reactivity
Rat, Mouse, Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Serum
Synonyms
SMAC/Diablo, Antibody; Polyclonal Antibody to SMAC/Diablo; DIABLO; SMAC; Direct IAP-binding protein with low pI; Smac; Second mitochondria-derived activator of caspase; Antibody to SMAC/Diablo; Anti-SMAC/Diablo Antibody; SMAC/Diablo Antibody; anti-DIABLO antibody
Host
Rabbit
Reactivity
Rat, Mouse, Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Serum
Form/Format
50 ul sera
Sequence Length
239
Applicable Applications for anti-DIABLO antibody
WB (Western Blot), IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen Information
A full-length recombinant protein of human SMAC was used as immunogen for this antibody
Antigen
SMAC/Diablo
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
IHC (Immunohistochemisry)
(Fig: 3 Formalin-fixed, paraffin-embedded human colon stained for Smac/DIABLO using at 1: 2000. A, normal colon. B and C, colon carcinoma. Only trace Smac/DIABLO immunoreactivity is seen in normal colonic epithelium (A), which is seen in the apical, foveolar cells. In contrast, strong immunoreactivity is observed in nuclear (B) and cytoplasmic (C) locations in the colon carcinoma specimen. Hematoxylin-eosin counterstain.)
IHC (Immunohistochemisry)
(Fig: 3 Formalin-fixed, paraffin-embedded human colon stained for Smac/DIABLO using at 1: 2000. A, normal colon. B and C, colon carcinoma. Only trace Smac/DIABLO immunoreactivity is seen in normal colonic epithelium (A), which is seen in the apical, foveolar cells. In contrast, strong immunoreactivity is observed in nuclear (B) and cytoplasmic (C) locations in the colon carcinoma specimen. Hematoxylin-eosin counterstain.)
IHC (Immunohiostchemistry)
(Fig: 2 Formalin-fixed, paraffin-embedded tissue section of human plasmacytoma (a mature B cell lymphoma) stained for Smac/DIABLO using at 1: 2000. Intense nuclear staining is observed. Hematoxylin-eosin counterstain)
IHC (Immunohiostchemistry)
(Fig: 2 Formalin-fixed, paraffin-embedded tissue section of human plasmacytoma (a mature B cell lymphoma) stained for Smac/DIABLO using at 1: 2000. Intense nuclear staining is observed. Hematoxylin-eosin counterstain)
WB (Western Blot)
(Fig: 1 Western blot analysis of Smac/DIABLO human tissues and cell lines using at 1: 2000. Legend. SMAC: full-length recombinant smac. RS1846 and Jurkat are tumor cell lines. All tissues are from normal donors except colon carcinoma (Colon Ca). SMAC expression was detected in all samples except spleen and normal colon.)
WB (Western Blot)
(Fig: 1 Western blot analysis of Smac/DIABLO human tissues and cell lines using at 1: 2000. Legend. SMAC: full-length recombinant smac. RS1846 and Jurkat are tumor cell lines. All tissues are from normal donors except colon carcinoma (Colon Ca). SMAC expression was detected in all samples except spleen and normal colon.)
Related Product Information for anti-DIABLO antibody
Smac/DIABLO (second mitochondrial-derived activator of caspase/direct IAP binding protein with low pI) is encoded by the nucleus and resides as a precursor within the intermembranous space of the mitochondria. During apoptosis, Smac/DIABLO is proteolytically cleaved and released from the mitochondria into the cytosol, where it promotes cell death by interacting with IAPs and inhibiting their anti-apoptotic function. In turn, certain IAPs may degrade Smac/DIABLO and block its cell death function. Smac/DIABLO is expressed in a wide range of normal tissues and some tumor cells lines, and its over-expression in tumor cell lines can trigger both extrinsic and intrinsic apoptosis pathways.
Product Categories/Family for anti-DIABLO antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
22,284 Da
NCBI Official Full Name
Diablo homolog, mitochondrial
NCBI Official Synonym Full Names
diablo IAP-binding mitochondrial protein
NCBI Official Symbol
DIABLO
NCBI Official Synonym Symbols
SMAC; DFNA64
NCBI Protein Information
diablo homolog, mitochondrial
UniProt Protein Name
Diablo homolog, mitochondrial
UniProt Gene Name
DIABLO
UniProt Synonym Gene Names
SMAC; Smac
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Product Notes
The DIABLO diablo (Catalog #AAA78356) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to SMAC/Diablo reacts with Rat, Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's SMAC/Diablo can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the DIABLO diablo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMAC/Diablo, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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