Standard Curve (Sample)
Human DNA-directed RNA polymerase II subunit RPB1, POLR2A ELISA Kit | POLR2A elisa kit
Human DNA-directed RNA polymerase II subunit RPB1, POLR2A ELISA Kit
Gene Names
POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
Reactivity
Human
Synonyms
DNA-directed RNA polymerase II subunit RPB1, POLR2A; N/A; Human DNA-directed RNA polymerase II subunit RPB1, POLR2A ELISA Kit; POLR2A elisa kit
Reactivity
Human
Sequence Length
1970
Samples
Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
15-3000ng/L
Sensitivity
8.12ng/L
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for POLR2A elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human DNA-directed RNA polymerase II subunit RPB1 (also known as POLR2A) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human POLR2A antibody. POLR2A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human POLR2A Antibody is added and binds to POLR2A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated POLR2A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human POLR2A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human POLR2A antibody. POLR2A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human POLR2A Antibody is added and binds to POLR2A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated POLR2A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human POLR2A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
DNA-directed RNA polymerase II subunit RPB1
NCBI Official Synonym Full Names
RNA polymerase II subunit A
NCBI Official Symbol
POLR2A
NCBI Official Synonym Symbols
RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
NCBI Protein Information
DNA-directed RNA polymerase II subunit RPB1
UniProt Protein Name
DNA-directed RNA polymerase II subunit RPB1
UniProt Gene Name
POLR2A
UniProt Synonym Gene Names
POLR2; RNA polymerase II subunit B1
UniProt Entry Name
RPB1_HUMAN
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Product Notes
The Human POLR2A polr2a (Catalog #AAA123513) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA123513 ELISA Kit recognizes Human POLR2A. It is sometimes possible for the material contained within the vial of "DNA-directed RNA polymerase II subunit RPB1, POLR2A, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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