Mouse RNA Polymerase II CTD Repeat YSPTSPS Monoclonal Antibody | anti-POLR2A antibody

RNA Polymerase II CTD Repeat YSPTSPS (Phospho S5)

Cat. Num. AAA215151

• Gene Names: POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
• Reactivity: Human, Saccharomyces cerevisiae
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA at 1.0mg/ml
• Synonyms: RNA Polymerase II CTD Repeat YSPTSPS, Antibody; RNA Polymerase II CTD Repeat YSPTSPS (Phospho S5); DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit RPB1; hRPB220; hsRPB1; POLR2; Polr2a; POLRA; Polymerase (RNA) II (DNA directed) polypeptide A 220kDa; RNA polymerase II subunit B1; RPBh1; RpIILS; RPO2; RPOL2; anti-POLR2A antibody

• Host: Mouse
• Reactivity: Human, Saccharomyces cerevisiae
• Clonality: Monoclonal
• Isotype: IgM, kappa
• Clone Number: CTD 8A7
• Specificity: RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 1970

• Applicable Applications for anti-POLR2A antibody: IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Positive Control: HAP1, K562, PC3, HePG2, NIH3T3 cells. Human testis.
• Immunogen: Ten repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5
• Cellular Localization: Nuclear
• Hu-chromosome Location: 17p13.1
• Preparation and Storage: Antibody with azide: store at 2 to 8°C.; Antibody without azide: store at -20 to -80°C.; Non-hazardous. No MSDS required.

WB (Western Blot)

(Western Blot Analysis of Human HepG2, K562 and SK-Br3 cell lysates using RNA Poll II Mouse Monoclonal Antibody (8A7).)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with RNA Polymerase II/Poll II Mouse Monoclonal Antibody (8A7).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with RNA Polymerase II/Poll II Mouse Monoclonal Antibody (8A7).)

References

Madak-Erdogan, Z., et al. 2008. Mol. Endocrinol. 22: 2116-2127

NCBI and Uniprot Product Information

• NCBI GI #: 4505939
• NCBI GeneID: 5430
• NCBI Accession #: NP_000928.1
• NCBI GenBank Nucleotide #: NP_000928.1
• UniProt Accession #: P24928
• Molecular Weight: 192-253kDa
• NCBI Official Full Name: DNA-directed RNA polymerase II subunit RPB1
• NCBI Official Synonym Full Names: RNA polymerase II subunit A
• NCBI Official Symbol: POLR2A
• NCBI Official Synonym Symbols: RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
• NCBI Protein Information: DNA-directed RNA polymerase II subunit RPB1
• UniProt Protein Name: DNA-directed RNA polymerase II subunit RPB1
• UniProt Gene Name: POLR2A
• UniProt Synonym Gene Names: POLR2; RNA polymerase II subunit B1
• UniProt Entry Name: RPB1_HUMAN