Standard Curve (Sample)
(Fig.1. Human Dual specificity protein phosphatase 6 (DUSP6) Standard Curve.)
Human Dual Specificity Protein Phosphatase 6 (DUSP6) ELISA Kit | DUSP6 elisa kit
Human Dual Specificity Protein Phosphatase 6 (DUSP6) ELISA Kit
Gene Names
DUSP6; HH19; MKP3; PYST1
Reactivity
Human
Synonyms
Dual Specificity Protein Phosphatase 6 (DUSP6); N/A; Human Dual Specificity Protein Phosphatase 6 (DUSP6) ELISA Kit; DUSP6; MKP3; PYST1; MAP kinase phosphatase 3; serine/threonine specific protein phosphatase; DUSP6 elisa kit
Reactivity
Human
Specificity
Human Dual specificity protein phosphatase 6 (DUSP6) ELISA Kit has high sensitivity and excellent specificity for detection of Human DUSP6. No significant cross-reactivity or interference between Human DUSP6 and analogues was observed.
Sequence Length
381
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Dual specificity protein phosphatase 6 (DUSP6) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Dual specificity protein phosphatase 6 (DUSP6) Standard Curve.)
Related Product Information for DUSP6 elisa kit
Description: This Human Dual specificity protein phosphatase 6 (DUSP6) ELISA Kit employs a two-site sandwich ELISA to quantitate DUSP6 in samples. An antibody specific for DUSP6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DUSP6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DUSP6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DUSP6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DUSP6 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily,which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm.
Background: DUSP6 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily,which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dual specificity protein phosphatase 6 isoform a
NCBI Official Synonym Full Names
dual specificity phosphatase 6
NCBI Official Symbol
DUSP6
NCBI Official Synonym Symbols
HH19; MKP3; PYST1
NCBI Protein Information
dual specificity protein phosphatase 6
UniProt Protein Name
Dual specificity protein phosphatase 6
UniProt Gene Name
DUSP6
UniProt Synonym Gene Names
MKP3; PYST1; MAP kinase phosphatase 3; MKP-3
UniProt Entry Name
DUS6_HUMAN
Similar Products
Product Notes
The Human DUSP6 dusp6 (Catalog #AAA331952) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331952 ELISA Kit recognizes Human DUSP6. It is sometimes possible for the material contained within the vial of "Dual Specificity Protein Phosphatase 6 (DUSP6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.