Standard Curve (Sample)
(Fig.1. Human E3 ubiquitin-protein ligase parkin (PARK2) Standard Curve.)
Human E3 Ubiquitin-Protein Ligase Parkin (PARK2) ELISA Kit | PARK2 elisa kit
Human E3 Ubiquitin-Protein Ligase Parkin (PARK2) ELISA Kit
Gene Names
PRKN; PDJ; AR-JP; LPRS2; PARK2
Reactivity
Human
Synonyms
E3 Ubiquitin-Protein Ligase Parkin (PARK2); N/A; Human E3 Ubiquitin-Protein Ligase Parkin (PARK2) ELISA Kit; PARK2; AR-JP; LPRS2; PDJ; PRKN; E3 ubiquitin ligase; parkin; parkin 2; PARK2 elisa kit
Reactivity
Human
Specificity
Human E3 ubiquitin-protein ligase parkin (PARK2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PARK2. No significant cross-reactivity or interference between Human PARK2 and analogues was observed.
Sequence Length
465
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human E3 ubiquitin-protein ligase parkin (PARK2) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human E3 ubiquitin-protein ligase parkin (PARK2) Standard Curve.)
Related Product Information for PARK2 elisa kit
Description: This Human E3 ubiquitin-protein ligase parkin (PARK2) ELISA Kit employs a two-site sandwich ELISA to quantitate PARK2 in samples. An antibody specific for PARK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PARK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PARK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PARK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Parkin is a proteinencoded by the PARK2 gene.The precise function of this protein is unknown; however, the protein is a component of a multiprotein E3 ubiquitin ligase complex which in turn is part of the ubiquitin-proteasome system that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause a familial form of Parkinson's disease known as autosomal recessive juvenile Parkinson disease. PARK2 is the parkin gene that may cause a form of autosomal recessive juvenile Parkinson disease due to a mutation in the parkin protein. This form of genetic mutation may be one of the most common known genetic causes of early-onset Parkinson disease.
Background: Parkin is a proteinencoded by the PARK2 gene.The precise function of this protein is unknown; however, the protein is a component of a multiprotein E3 ubiquitin ligase complex which in turn is part of the ubiquitin-proteasome system that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause a familial form of Parkinson's disease known as autosomal recessive juvenile Parkinson disease. PARK2 is the parkin gene that may cause a form of autosomal recessive juvenile Parkinson disease due to a mutation in the parkin protein. This form of genetic mutation may be one of the most common known genetic causes of early-onset Parkinson disease.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
E3 ubiquitin-protein ligase parkin isoform 1
NCBI Official Synonym Full Names
parkin RBR E3 ubiquitin protein ligase
NCBI Official Symbol
PRKN
NCBI Official Synonym Symbols
PDJ; AR-JP; LPRS2; PARK2
NCBI Protein Information
E3 ubiquitin-protein ligase parkin
UniProt Protein Name
E3 ubiquitin-protein ligase parkin
UniProt Gene Name
PARK2
UniProt Synonym Gene Names
PRKN; Parkinson disease protein 2
UniProt Entry Name
PRKN2_HUMAN
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Product Notes
The Human PARK2 park2 (Catalog #AAA331470) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331470 ELISA Kit recognizes Human PARK2. It is sometimes possible for the material contained within the vial of "E3 Ubiquitin-Protein Ligase Parkin (PARK2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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