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product-image-AAA143426_IHC11.jpg IHC (Immunohistochemisry) (DAB staining on IHC-P. Samples: Mouse Tissue))

Rabbit anti-Mouse Epidermal Growth Factor Receptor Polyclonal Antibody | anti-EGFR antibody

Polyclonal Antibody to Epidermal Growth Factor Receptor (EGFR)

Gene Names
Egfr; Wa5; wa2; Erbb; Errp; wa-2; Errb1; AI552599; 9030024J15Rik
Reactivity
Mouse
Applications
ELISA, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Affinity Chromatography
Synonyms
Epidermal Growth Factor Receptor, Antibody; Polyclonal Antibody to Epidermal Growth Factor Receptor (EGFR); anti-EGFR antibody
Ordering
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against EGFR. It has beenselected for its ability to recognize EGFR in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- EE DGIRKCKKCD GPCRKVCNGI GIGEFKDTLS INATNIKHFKYCTAISGDLH ILPVAFKGDS FTRTPPLDPR ELEILKTVKE ITGFLLIQAW PDNWTDLHAFENLEIIRGRT KQHGQFSLAV VGLNITSLGL RSLKEISDGD VIISGNRNLC YANTINWKKLFGTP
Sequence Length
10
Applicable Applications for anti-EGFR antibody
ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
Fragment
EGFR (Glu319~Pro484)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse
Conjugate
No Conjugate
Immunogen
Recombinant EGFR (Glu319~Pro484) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

IHC (Immunohistochemisry)

(DAB staining on IHC-P. Samples: Mouse Tissue))

product-image-AAA143426_IHC11.jpg IHC (Immunohistochemisry) (DAB staining on IHC-P. Samples: Mouse Tissue))

WB (Western Blot)

(Western Blot: Sample: Recombinant protein.)

product-image-AAA143426_WB13.jpg WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

WB (Western Blot)

(Western Blot: Sample: Mouse Serum; Primary Ab: 1ug/ml Rabbit Anti-Mouse EGFR Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

product-image-AAA143426_WB15.jpg WB (Western Blot) (Western Blot: Sample: Mouse Serum; Primary Ab: 1ug/ml Rabbit Anti-Mouse EGFR Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
134,853 Da
NCBI Official Full Name
epidermal growth factor receptor isoform 1
NCBI Official Synonym Full Names
epidermal growth factor receptor
NCBI Official Symbol
Egfr
NCBI Official Synonym Symbols
Wa5; wa2; Erbb; Errp; wa-2; Errb1; AI552599; 9030024J15Rik
NCBI Protein Information
epidermal growth factor receptor
UniProt Protein Name
Epidermal growth factor receptor
UniProt Gene Name
Egfr

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Product Notes

The EGFR egfr (Catalog #AAA143426) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Epidermal Growth Factor Receptor (EGFR) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Epidermal Growth Factor Receptor can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the EGFR egfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- EE DGIRKCKKCD GPCRKVCNGI GIGEFKDTLS INATNIKHFK YCTAISGDLH ILPVAFKGDS FTRTPPLDPR ELEILKTVKE ITGFLLIQAW PDNWTDLHAF ENLEIIRGRT KQHGQFSLAV VGLNITSLGL RSLKEISDGD VIISGNRNLC YANTINWKKL FGTP. It is sometimes possible for the material contained within the vial of "Epidermal Growth Factor Receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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