Standard Curve (Sample)
Human Epididymal secretory protein E1 (NPC2) ELISA Kit | NPC2 elisa kit
Human Epididymal secretory protein E1 (NPC2) ELISA Kit
Gene Names
NPC2; HE1; EDDM1
Reactivity
Human
Synonyms
Epididymal secretory protein E1 (NPC2); N/A; Human Epididymal secretory protein E1 (NPC2) ELISA Kit; NPC2 elisa kit
Reactivity
Human
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for NPC2 elisa kit
Intended Uses: This NPC2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NPC2. This ELISA kit for research use only!
Principle of the Assay: NPC2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-NPC2 antibody and an NPC2-HRP conjugate. The assay sample and buffer are incubated together with NPC2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NPC2 concentration since NPC2 from samples and NPC2-HRP conjugate compete for the anti-NPC2 antibody binding site. Since the number of sites is limited, as more sites are occupied by NPC2 from the sample, fewer sites are left to bind NPC2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NPC2 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: NPC2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-NPC2 antibody and an NPC2-HRP conjugate. The assay sample and buffer are incubated together with NPC2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NPC2 concentration since NPC2 from samples and NPC2-HRP conjugate compete for the anti-NPC2 antibody binding site. Since the number of sites is limited, as more sites are occupied by NPC2 from the sample, fewer sites are left to bind NPC2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NPC2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,570 Da
NCBI Official Full Name
epididymal secretory protein E1
NCBI Official Synonym Full Names
Niemann-Pick disease, type C2
NCBI Official Symbol
NPC2
NCBI Official Synonym Symbols
HE1; EDDM1
NCBI Protein Information
epididymal secretory protein E1; epididymal protein 1; tissue-specific secretory protein; human epididymis-specific protein 1; Niemann-Pick disease type C2 protein
UniProt Protein Name
Epididymal secretory protein E1
UniProt Gene Name
NPC2
UniProt Synonym Gene Names
HE1; He1
UniProt Entry Name
NPC2_HUMAN
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Product Notes
The Human NPC2 npc2 (Catalog #AAA245617) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA245617 ELISA Kit recognizes Human NPC2. It is sometimes possible for the material contained within the vial of "Epididymal secretory protein E1 (NPC2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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