Standard Curve (Sample)
Human ERG (Transcriptional regulator ERG) ELISA Kit | ERG elisa kit
Human ERG (Transcriptional regulator ERG) ELISA Kit
Gene Names
ERG; p55; erg-3
Reactivity
Human
Synonyms
ERG (Transcriptional regulator ERG); N/A; Human ERG (Transcriptional regulator ERG) ELISA Kit; Transcriptional regulator ERG; Transforming protein ERG; ERG; ERG elisa kit
Reactivity
Human
Specificity
Specifically recognize ERG, no obvious cross reaction with other analogues
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
78.125-5000pg/ml
Sensitivity
46.875pg/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ERG elisa kit
Background: ETS-related gene (ERG) is a member of the E-26 transformation-specific (ETS) family of sequence-specific DNAbinding transcription factors. ERG plays important and highly conserved roles in vertebrate development. Early in embryonic development, ERG is highly expressed in the embryonic mesoderm and endothelium, where it plays a critical role in the formation of the vascular system, urogenital tract and bone development. Later in embryonic development, ERG functions to regulate the pluripotency of hematopoietic stem cells, endothelial cell homeostasis and angiogenesis.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti ERG antibody was precoated onto the 96-well plate. The biotin conjugated anti ERG antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with ERG conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of ERG in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti ERG antibody was precoated onto the 96-well plate. The biotin conjugated anti ERG antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with ERG conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of ERG in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
486
NCBI Official Full Name
Transcriptional regulator ERG
NCBI Official Synonym Full Names
v-ets avian erythroblastosis virus E26 oncogene homolog
NCBI Official Symbol
ERG
NCBI Official Synonym Symbols
p55; erg-3
NCBI Protein Information
transcriptional regulator ERG; ets-related; TMPRSS2/ERG fusion; v-ets erythroblastosis virus E26 oncogene like; v-ets erythroblastosis virus E26 oncogene homolog; v-ets avian erythroblastosis virus E26 oncogene related; transcriptional regulator ERG (tran
UniProt Protein Name
Transcriptional regulator ERG
UniProt Gene Name
ERG
UniProt Entry Name
ERG_HUMAN
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Product Notes
The Human ERG erg (Catalog #AAA27637) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA27637 ELISA Kit recognizes Human ERG. It is sometimes possible for the material contained within the vial of "ERG (Transcriptional regulator ERG), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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