Human Fas ELISA Kit | Fas elisa kit

Human Fas ELISA Kit

Cat. Num. AAA258346

• Reactivity: Human
• Synonyms: Fas; N/A; Human Fas ELISA Kit; Fas/CD95 ELISA Kit, Human; Fas cell surface death receptor; ALPS1A ELISA Kit, Human; APO-1 ELISA Kit, Human; APT1 ELISA Kit, Human; CD95 ELISA Kit, Human; FAS1 ELISA Kit, Human; FASTM ELISA Kit, Human; TNFRSF6 ELISA Kit, Human; Fas elisa kit

• Reactivity: Human
• Specificity: For the quantitative determination of Human Fas concentration in serum, cell culture supernates and plasma.
• Assay Type: Solid Phase Sandwich ELISA (quantitative)
• Sample Types: Serum, Cell supernatant, Plasma
• Detection Range: 25-1600pg/mL
• Sensitivity: 14.6pg/mL
• Conjugation: HRP
• Interference: Preparations of the factors listed below at 50ng/mL in a mid-range recombinant human FAS control were assayed for interference. No significant interference was observed.
• Materials Provided: 1. 96 well microplate coated with Capture Antibody; 2. Detection Antibody conjugated to HRP; 3. Standards; 4. Wash Buffer Concentrate; 5. Dilution Buffer Concentrate; 6. Color Reagent A; 7. Color Reagent B; 8. Stop Solution

Application Data

(This assay recognizes both recombinant and natural Human FAS. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)

Standard Curve (Sample)

(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)

Related Product Information for Fas elisa kit

This Fas/CD95 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Fas/CD95 protein in Serum, Cell supernatant, Plasma. It contains recombinant Human Fas/CD95, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.

Background: CD95 (APO-1/Fas) is an important inducer of the extrinsic apoptosis signaling pathway and therapy induced apoptosis of many tumor cells has been linked to the activity of CD95. is a prototype death receptor characterized by the presence of an 80 amino acid death domain in its cytoplasmic tail. This domain is essential for the recruitment of a number of signaling components upon activation by either agonistic anti-CD95 antibodies or cognate CD95 ligand that initiate apoptosis. The complex of proteins that forms upon triggering of CD95 is called the death-inducting signaling complex (DISC). The DISC consists of an adaptor protein and initiator caspases and is essential for induction of apoptosis. CD95 is also crucial for the negative selection of B cells within the germinal center (GC). Impairment of CD95-mediated apoptosis results in defective affinity maturation and the persistence of autoreactive B-cell clones. Changes in the expression of CD95 and/or its ligand CD95L are frequently found in human cancer. The downregulation or mutation of CD95 has been proposed as a mechanism by which cancer cells avoid destruction by the immune system through reduced apoptosis sensitivity. Thus, CD95 has therefore been viewed as a tumor suppressor. CD95 has been reported to be involved in the activation of NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and the alternate pathways for CTL-mediated cytotoxicity. Accordingly, this protein is implicated in the pathogenesis of various malignancies and diseases of the immune system. The CD95/CD95L system was implicated in the etiology of inflammatory bowel disease (IBD) based, primarily, on the finding that CD95 is highly expressed in the intestinal epithelial cells and that epithelial apoptosis is increased in IBD.

References

Mschen M, et al. (2002) The origin of CD95-gene mutations in B-cell lymphoma. Trends Immunol. 23(2): 75-80.
Peter ME, et al. (2003) The CD95(APO-1/Fas) DISC and beyond. Cell Death Differ. 10(1): 26-35.
Peter ME, et al. (2005) Does CD95 have tumor promoting activities Biochim Biophys Acta. 1755(1): 25-36.
Chen L, et al. (2010) Cell death in the colonic epithelium during inflammatory bowel diseases: CD95/Fas and beyond. Inflamm Bowel Dis. 16(6): 1071-6.