Standard Curve (Sample)
Human Folate ELISA Kit
Folate ELISA
Reactivity
Human
Synonyms
Folate; N/A; Folate ELISA; Folate elisa kit
Reactivity
Human
Assay Type
Quantitative Competitive
Samples
Human serum and plasma
Preparation and Storage
Store at 2-8 degree C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Folate elisa kit
Principle of the Assay: The Folate test kit is a solid phase enzyme-linked immunoassay (ELISA), based on the principal of competitive binding. Streptavidin coated wells are co-incubated with extracted Folate standards, controls, samples, Folate Binding Protein-Biotin conjugate and Folate-Enzyme conjugate at room temperature for 60 minutes. During the incubation, the folate-enzyme conjugate and the folate in the sample, standard, or quality control serum will compete to bind to the biotin-labeled folate binding protein. After the 60 minute incubation, all unbound conjugates are then removed and the wells are washed. Next, a solution of TMB Reagent is added and incubated at room temperature for 15 minutes, resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is measured spectrophotometrically at 450 nm. A standard curve is obtained by plotting the concentration of the standard versus the absorbance. The color intensity is inversely proportional to the amount of Folate in the sample. The total assay procedure run time is 1.25 hours.
Intended Uses: The Folate ELISA test kit is intended for the quantitative determination of Folic acid in human serum and plasma. For Research Use Only. Not for use in Test Procedures.
Intended Uses: The Folate ELISA test kit is intended for the quantitative determination of Folic acid in human serum and plasma. For Research Use Only. Not for use in Test Procedures.
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Product Notes
The Human Folate (Catalog #AAA72730) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA72730 ELISA Kit recognizes Human Folate. It is sometimes possible for the material contained within the vial of "Folate, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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