Standard Curve (Sample)
Human Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) ELISA Kit | GRIN1 elisa kit
Human Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) ELISA Kit
Gene Names
GRIN1; NR1; MRD8; GluN1; NMDA1; NDHMSD; NDHMSR; NMD-R1; NMDAR1
Reactivity
Human
Synonyms
Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1); N/A; Human Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) ELISA Kit; NMDA1; NMDAR1; NR1; Glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; GRIN1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of GRIN1. No significant cross-reactivity or interference between GRIN1 and analogues was observed.
Sequence Length
885
Samples
Tissue homogenates, cell lysates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
<0.056ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GRIN1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GRIN1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for GRIN1 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of GRIN1 in human tissue homogenates, cell lysates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GRIN1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GRIN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GRIN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GRIN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GRIN1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GRIN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GRIN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GRIN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
101,851 Da
NCBI Official Full Name
glutamate receptor ionotropic, NMDA 1 isoform GluN1-4a
NCBI Official Synonym Full Names
glutamate ionotropic receptor NMDA type subunit 1
NCBI Official Symbol
GRIN1
NCBI Official Synonym Symbols
NR1; MRD8; GluN1; NMDA1; NDHMSD; NDHMSR; NMD-R1; NMDAR1
NCBI Protein Information
glutamate receptor ionotropic, NMDA 1
UniProt Protein Name
Glutamate receptor ionotropic, NMDA 1
UniProt Gene Name
GRIN1
UniProt Synonym Gene Names
NMDAR1; GluN1; NMD-R1
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Product Notes
The Human GRIN1 grin1 (Catalog #AAA68564) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA68564 ELISA Kit recognizes Human GRIN1. It is sometimes possible for the material contained within the vial of "Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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