Mouse HSP27 Monoclonal Antibody | anti-HSP27 antibody

HSP27 monoclonal antibody (G3.1)

Cat. Num. AAA77022

• Gene Names: HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
• Reactivity: Human, Mouse, Rat, Monkey, Bovine, Fish
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, ELISA
• Purity: Protein G affinity purified.
• Synonyms: HSP27, Antibody; HSP27 monoclonal antibody (G3.1); HspB1; Heat shock protein 27; HSP27, mAb (G3.1); anti-HSP27 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Monkey, Bovine, Fish
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: G3.1
• Specificity: Recognizes human, mouse, rat, bovine, fish, and monkey HSP27. Detects a band of ~27kDa by Western blot.
• Purity/Purification: Protein G affinity purified.
• Form/Format: Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
• Sequence Length: 205

• Applicable Applications for anti-HSP27 antibody: EM (Electron Microscopy), WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), ICC (Immunocytochemistry), ELISA
• Immunogen: Native human Hsp27.
• Preparation and Storage: Long Term: -20 degree C; Avoid freeze/thaw cycles.

WB (Western Blot)

(Western blot analysis of HSP27, mAb (G3.1). Lane 1: MW marker; Lane 2: HeLa (heat shocked); Lane 3: Vero (heat shocked), Lane 4: HSP27 (human) (recombinant).)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of human skeletal muscle tissue stained with HSP27, mAb (G3.1) at 10µg/ml.)

Related Product Information for anti-HSP27 antibody

Hsp27 is one of the most common members of the highly conserved and ubiquitously expressed family of small heat shock proteins (sHsp), which also includes alphaB-crystallin. It is characterized by a conserved C-terminal alpha-crystallin domain consisting of two anti-parallel beta-sheets that promote oligomer formation required for its primary chaperone function as inhibitor of irreversible protein aggregation. Hsp27 oligomerization is modulated by post-translational phosphorylation of Hsp27 at three serine residues, Ser15, Ser78, and Ser82, by a variety of protein kinases including MAPKAPK-3, PKAc-alpha, p70 S6K, PKD I, and PKC-delta. Hsp27 has been shown to inhibit actin polymerization by binding of unphosphorylated Hsp27 monomers to actin intermediate filaments. Anti-apoptotic functions of Hsp27 have also been identified through interactions with DAXX7, activation of Akt, and inhibition of apoptosome formation. Evidence suggests altered expression of Hsp27 is implicated in the pathogenesis of breast, ovarian, and prostate cancer.

Product Categories/Family for anti-HSP27 antibody

Protein Synthesis & Folding

NCBI and Uniprot Product Information

• NCBI GI #: 19855073
• NCBI GeneID: 3315
• UniProt Accession #: P04792; Q6FI47; Q96C20; Q96EI7; Q9UC31; Q9UC34; Q9UC35; Q9UC36; B2R4N8
• Molecular Weight: 22,783 Da
• NCBI Official Full Name: Heat shock protein beta-1
• NCBI Official Synonym Full Names: heat shock protein family B (small) member 1
• NCBI Official Symbol: HSPB1
• NCBI Official Synonym Symbols: CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
• NCBI Protein Information: heat shock protein beta-1
• UniProt Protein Name: Heat shock protein beta-1
• UniProt Gene Name: HSPB1
• UniProt Synonym Gene Names: HSP27; HSP28; HspB1; HSP 27; SRP27