Standard Curve (Sample)
(Human IFNA2 PicoKine ELISA Kit standard curve)
Human IFNA2 ELISA Kit | IFNA2 elisa kit
Human IFNA2 ELISA Kit PicoKine
Gene Names
IFNA2; IFNA; INFA2; IFNA2B; IFN-alphaA
Reactivity
Human
Applications
ELISA
Synonyms
IFNA2; N/A; Human IFNA2 ELISA Kit PicoKine; Interferon gamma; IFN-gamma; Immune interferon; IFNG; interferon alpha 2; IFNA2 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human IFNA2
Samples
Cell culture supernatants and serum
Detection Range
3.12 pg/ml - 200 pg/ml
Sensitivity
<1 pg/ml
Applicable Applications for IFNA2 elisa kit
ELISA
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Standard Curve (Sample)
(Human IFNA2 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human IFNA2 PicoKine ELISA Kit standard curve)
Related Product Information for IFNA2 elisa kit
Background: Interferon alpha-2 is a protein that in humans is encoded by the IFNA2 gene. This gene is a member of the alpha interferon gene cluster on chromosome 9. The encoded cytokine is a member of the type I interferon family that is produced in response to viral infection as a key part of the innate immune response with potent antiviral, antiproliferative and immunomodulatory properties. This cytokine, like other type I interferons, binds a plasma membrane receptor made of IFNAR1 and IFNAR2 that is ubiquitously expressed, and thus is able to act on virtually all body cells. The encoded protein is effective in reducing the symptoms and duration of the common cold and in treating many types of cancer, including some hematological malignancies and solid tumors. A deficiency of type I interferon in the blood is thought to be a hallmark of severe COVID-19 and may provide a rationale for a combined therapeutic approach.
Principle of the Assay: The Picokine™ Human IFNA2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human IFNA2 with a 96-well strip plate that is pre-coated with antibody specific for IFNA2. The detection antibody is a biotinylated antibody specific for IFNA2. The capture antibody is a monoclonal antibody from mouse and the detection antibody is a biotinylated polyclonal antibody from goat. The kit includes Human IFNA2 protein as standards. To measure Human IFNA2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human IFNA2 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human IFNA2 in the sample.
Principle of the Assay: The Picokine™ Human IFNA2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human IFNA2 with a 96-well strip plate that is pre-coated with antibody specific for IFNA2. The detection antibody is a biotinylated antibody specific for IFNA2. The capture antibody is a monoclonal antibody from mouse and the detection antibody is a biotinylated polyclonal antibody from goat. The kit includes Human IFNA2 protein as standards. To measure Human IFNA2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human IFNA2 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human IFNA2 in the sample.
Product Categories/Family for IFNA2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,550 Da
NCBI Official Full Name
interferon alpha-2
NCBI Official Synonym Full Names
interferon, alpha 2
NCBI Official Symbol
IFNA2
NCBI Official Synonym Symbols
IFNA; INFA2; IFNA2B; IFN-alphaA
NCBI Protein Information
interferon alpha-2; IFN-alpha-2; alpha-2a interferon; interferon alpha 2a; interferon alpha 2b; interferon alpha A; leIF A
UniProt Protein Name
Interferon alpha-2
UniProt Gene Name
IFNA2
UniProt Synonym Gene Names
IFNA2A; IFNA2B; IFNA2C; IFN-alpha-2; LeIF A
UniProt Entry Name
IFNA2_HUMAN
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Product Notes
The Human IFNA2 ifna2 (Catalog #AAA126677) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA126677 ELISA Kit recognizes Human IFNA2. AAA Biotech's IFNA2 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the IFNA2 ifna2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IFNA2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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