Human IGFBP4 PicoKine ELISA Kit | IGFBP4 elisa kit

Human IGFBP4 PicoKine ELISA Kit

Cat. Num. AAA46148

• Gene Names: IGFBP4; BP-4; IBP4; IGFBP-4; HT29-IGFBP
• Reactivity: Human
• Synonyms: IGFBP4 PicoKine; N/A; Human IGFBP4 PicoKine ELISA Kit; Insulin-like growth factor-binding protein 4; AI875747; BP 4; BP4; Deb2; HT29 IGFBP; IBP 4; IBP-4; IBP4; IBP4_HUMAN; IGF binding protein 4; IGF-binding protein 4; IGF-BP4; IGFBP 4; IGFBP-4; IGFBP4; Insulin Like Growth Factor Binding Protein 4; Insulin-like growth factor-binding protein 4 ; insulin-like growth factor binding protein 4; IGFBP4 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human IGFBP4
• Sequence Length: 258
• Assay Range: 500pg/ml-32000pg/ml
• Sensitivity: <20pg/ml
• Sample: For quantitative detection of human IGFBP4 in cell culture supernates, serum, and plasma(heparin, EDTA).

• Immunogen: NSO, D22-E258
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 32000pg/ml, 16000pg/ml, 8000pg/ml, 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml human IGFBP4 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IGFBP4 standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|846|3152|12,519|755|3646|13698; Standard deviation|43.99|179.66|688.54|49.83|258.86|876.67; CV(%)|5.2|5.7|5.5|6.6|7.1|6.4
• Sample Data: Concentration(pg/ml)|0|500|1000|2000|4000|8000|16000|32000; O.D.|0.031|0.169|0.200|0.280|0.401|0.660|1.140|1.880
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Standard Curve (Sample)

(Human IGFBP4 PicoKine ELISA Kit standard curve)

Related Product Information for IGFBP4 elisa kit

Principle of the assay: Human IGFBP4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP4 has been precoated onto 96-well plates. Standards (NSO, D22-E258) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGFBP4 amount of sample captured in plate.

Background: Insulin-like growth factor-binding protein 4 is a protein that in humans is encoded by the IGFBP4gene. This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. In addition, IGFBP-4 is a unique protein and it consistently inhibits several cancer cells in vivo and in vitro. Its inhibitory action has been shown in vivo in prostate and colon. It is secreted by all colon cancer cells.

NCBI and Uniprot Product Information

• NCBI GI #: 62243290
• NCBI GeneID: 3487
• NCBI Accession #: NP_001543.2
• NCBI GenBank Nucleotide #: NM_001552.2
• UniProt Accession #: P22692; Q5U012; Q9UCL6; A0N9W2; B4E351
• Molecular Weight: 17,635 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 4
• NCBI Official Synonym Full Names: insulin like growth factor binding protein 4
• NCBI Official Symbol: IGFBP4
• NCBI Official Synonym Symbols: BP-4; IBP4; IGFBP-4; HT29-IGFBP
• NCBI Protein Information: insulin-like growth factor-binding protein 4
• UniProt Protein Name: Insulin-like growth factor-binding protein 4
• UniProt Gene Name: IGFBP4
• UniProt Synonym Gene Names: IBP4; IBP-4; IGF-binding protein 4; IGFBP-4
• UniProt Entry Name: IBP4_HUMAN