Standard Curve (Sample)
IL-1 beta elisa kit
Human IL-1 beta ELISA Kit
Gene Names
IL1B; IL-1; IL1F2; IL1-BETA
Reactivity
Human. No cross reactivity with other human cytokines.
Synonyms
IL-1 beta; N/A; Human IL-1 beta ELISA Kit; IL-1 b; IL-1 beta elisa kit
Reactivity
Human. No cross reactivity with other human cytokines.
Specificity
The assay recognizes both natural and recombinant human IL-1beta. To define the specificity of this ELISA several proteins were tested for cross reactivity. There was no cross reactivity observed for any protein tested (IL-1alpha, IL-2, IL-10, IL-12, IL-17A, IL-23, IFNgamma, Gp130, IL-33, TNFalpha).
Sequence Length
269
Samples
Serum and plasma samples
Assay Type
Quantitative Sandwich
Sensitivity
6.5 pg/ml.
Intra-assay Precision
Reproducibility within the assay was evaluated in three independent experiments. Each assay was carried out with 6 replicates (3 duplicates) in 2 human pooled serum, 2 in RPMI and 2 in standard diluent with samples containing different concentrations of IL-1beta. 2 standard curves were run on each plate. The overall intra-assay coefficient of variation has been calculated to be 4.5%
Inter-assay Precision
Assay to assay reproducibility within one laboratory was evaluated in three independent experiments by two technicians. Each assay was carried out with 6 replicates (3 duplicates) in 2 human pooled serum, 2 in RPMI and 2 in standard diluent with samples containing different concentrations of IL-1beta. 2 standard curves were run on each plate. The calculated overall coefficient of variation was 8.7%.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IL-1 beta elisa kit
Intended Uses: IL-1beta ELISA kit is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of IL-1beta in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant human IL-1beta.
Principle of the Assay: A capture Antibody highly specific for IL-1beta has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of IL-1beta samples and known standards to the capture antibodies and subsequent binding of the biotinylated anti IL-1beta secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of IL-1beta present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of IL-1beta in any sample tested.
Principle of the Assay: A capture Antibody highly specific for IL-1beta has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of IL-1beta samples and known standards to the capture antibodies and subsequent binding of the biotinylated anti IL-1beta secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of IL-1beta present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of IL-1beta in any sample tested.
Product Categories/Family for IL-1 beta elisa kit
References
Beyth S. et al., Blood, 2005; 105(5): 2214 - 2219
Buenestado, A. et al., J Parenter Enteral Nutr.,2006;30(4):286-96
Cunin, P. et al., J. Immunol.,2011;186(7): 4175-4182.
Buenestado, A. et al., J Parenter Enteral Nutr.,2006;30(4):286-96
Cunin, P. et al., J. Immunol.,2011;186(7): 4175-4182.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,748 Da
NCBI Official Full Name
interleukin-1 beta proprotein
NCBI Official Synonym Full Names
interleukin 1, beta
NCBI Official Symbol
IL1B
NCBI Official Synonym Symbols
IL-1; IL1F2; IL1-BETA
NCBI Protein Information
interleukin-1 beta
UniProt Protein Name
Interleukin-1 beta
UniProt Gene Name
IL1B
UniProt Synonym Gene Names
IL1F2; IL-1 beta
UniProt Entry Name
IL1B_HUMAN
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Product Notes
The Human. No cross reactivity with other human cytokines. IL-1 beta il1b (Catalog #AAA58604) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA58604 ELISA Kit recognizes Human. No cross reactivity with other human cytokines. IL-1 beta. It is sometimes possible for the material contained within the vial of "IL-1 beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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