Human IL-10 ELISA Kit | IL-10 elisa kit

Human IL-10 ELISA Kit (High Sensitivity)

Cat. Num. AAA269043

• Reactivity: Human
• Synonyms: IL-10; N/A; Human IL-10 ELISA Kit (High Sensitivity); IL-10 elisa kit

• Reactivity: Human
• Specificity: Not compatible with IL-10Ralpha, IL-10R beta/Fc Chimera, Recombinant mouse IL-10 and IL-10Ralpha, Recombinant mouse EQUine IL-10, Feline il-10Il-10, Porcine IL-10, rat IL-10, etc.
• Assay Type: Sandwich
• Samples: Cell culture supernates, serum, and plasma
• Sensitivity: 0.15 pg/mL

• Preparation and Storage: Store at 4 degree C; Shipping: Gel Ice

Standard Curve (Sample)

Related Product Information for IL-10 elisa kit

Background: Interleukin 10 (IL-10) is mainly produced by Th2 cells and is an immune regulatory cytokine that inhibits the release of cytokines (IFN-gamma and IL-2, etc.) by Th1 cells.Il-10 can also be produced by other cells and has many other functions.Cytokine synthesis inhibitory factor (CSIF), B cell-derived T cell growth factor, and cytokine cytokine synthesis inhibitory factor (CSIF).B - TCGF), etc.Il-10 mainly targets T cells, NK cells, B cells, mononuclear macrophages and mast cells and exerts inhibitory effects.The effect of IL-10 on T cells is biphasic.IL - 10 inhibition of CD4+T cells synthesize and produce IL-2, IL-5, TNF-A and IFN-gamma.Il-10 inhibited THE release of IFN-gamma by CTL.Il-10 also inhibits IFN-gamma production in NK cells, which is different from the inhibitory effect of IL-4.Il-4 directly inhibits NK cells;Il-10 works through mononuclear macrophages.Il-10 is an auxiliary growth factor for T cell development in mature, immature and thymus cells.Il-10 stimulates the rapid growth and differentiation of human B cells activated by anti-CD40 antibodies (or antigens) and produces large amounts of IgG, IgA, and IgM.Il-10 did not support the growth of mast cells or mast cell precursors, but significantly increased the growth-promoting effect of IL-3 or IL-4 on mouse mast cells.Il-10 inhibited macrophage antigen presentation to Th1 cells, and reduced macrophage expression of MHC-II molecules, ICAM-1 and B7 molecules.Il-10 inhibits cytokine synthesis by macrophages.

Principle of the Assay: ELISA kit uses double antibody sandwich method: anti-human IL-10 monoclonal antibody is coated on a plate, and human IL-10 is bound to monoclonal antibody in specimens and standard products, and the free components are washed away. Biotinylated anti-human IL-10 antibody and horseradish peroxidase labeled avidin were added, and biotin specifically bound to avidin;Anti-human IL-10 antibodies bind to human IL-10 bound to a single antibody to form an immune complex in which the free components are washed away.Add color substrate, if there is IL-10 in the reaction hole, horseradish peroxidase will make the colorless color agent blue, add stop solution to turn yellow.OD measurement at 450 nm shows that the concentration of HUMAN IL-10 is in direct proportion to OD450. The concentration of HUMAN IL-10 can be calculated by drawing a standard curve.