Standard Curve (Sample)
(Human IL-17RA PicoKine ELISA Kit standard curve)
Human IL-17RA PicoKine ELISA Kit | IL17RA elisa kit
Human IL-17RA PicoKine ELISA Kit
Gene Names
IL17RA; CD217; IL17R; CANDF5; CDw217; IL-17RA; hIL-17R
Reactivity
Human
Synonyms
IL-17RA PicoKine; N/A; Human IL-17RA PicoKine ELISA Kit; Interleukin-17 receptor A; CANDF5; CD217; CD217 antigen; CDw217; CTLA8; HIL 17R; I17RA_HUMAN; IL 17 receptor A; IL 17 receptor; IL 17RA; IL-17 receptor A; IL-17RA; IL17; IL17A receptor; IL17R; IL17RA; Interleukin 17 receptor A; MGC10262 ; interleukin 17 receptor A; IL17RA elisa kit
Reactivity
Human
Specificity
Natural and recombinant human IL-17RA
Sequence Length
832
Assay Range
31.2pg/ml-2000pg/ml
Sensitivity
<10pg/ml
Sample
For quantitative detection of human IL-17RA in cell culture supernates, serum and plasma(heparin, EDTA).
Immunogen
NSO, L33-W320
Assay Procedure Step 1
Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human IL-17RA standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum and plasma (heparin, EDTA) to each empty well. It is recommended that each human IL-17RA standard solution and each sample be measured in duplicate.
Intra-Assay Precision
Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|258|762|1229|262|770|1335
Standard deviation|17.02|44.95|70.05|18.34|49.28|82.77
CV(%)|6.6|5.9|5.7|7.0|6.4|6.2
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|258|762|1229|262|770|1335
Standard deviation|17.02|44.95|70.05|18.34|49.28|82.77
CV(%)|6.6|5.9|5.7|7.0|6.4|6.2
Sample Data
Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000
O.D.|0.033|0.108|0.182|0.287|0.531|0.912|1.613|2.116
O.D.|0.033|0.108|0.182|0.287|0.531|0.912|1.613|2.116
Anticoagulant
Heparin or EDTA
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Standard Curve (Sample)
(Human IL-17RA PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human IL-17RA PicoKine ELISA Kit standard curve)
Related Product Information for IL17RA elisa kit
Principle of the assay: Human IL-17RA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-17RA has been precoated onto 96-well plates. Standards (NSO, L33- W320) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17RA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-17RA amount of sample captured in plate.
Background: Interleukin 17 receptor A, also known as IL17RA and CDw217 (cluster of differentiation w217), is a human gene. The IL17R gene is mapped to 22q11.22-q11.23 by radiation hybrid analysis. Interleukin 17A (IL17A) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. The protein encoded by this gene is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Like other cytokine receptors, this receptor likely has a multimeric structure. Also, it is showed that mouse Il17r bound HSV-13 and mouse Il17. Recombinant soluble Il17r inhibited mitogen-induced T-cell proliferation and Il2 production.
Background: Interleukin 17 receptor A, also known as IL17RA and CDw217 (cluster of differentiation w217), is a human gene. The IL17R gene is mapped to 22q11.22-q11.23 by radiation hybrid analysis. Interleukin 17A (IL17A) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. The protein encoded by this gene is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Like other cytokine receptors, this receptor likely has a multimeric structure. Also, it is showed that mouse Il17r bound HSV-13 and mouse Il17. Recombinant soluble Il17r inhibited mitogen-induced T-cell proliferation and Il2 production.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
92,153 Da
NCBI Official Full Name
interleukin-17 receptor A isoform 2
NCBI Official Synonym Full Names
interleukin 17 receptor A
NCBI Official Symbol
IL17RA
NCBI Official Synonym Symbols
CD217; IL17R; CANDF5; CDw217; IL-17RA; hIL-17R
NCBI Protein Information
interleukin-17 receptor A
UniProt Protein Name
Interleukin-17 receptor A
UniProt Gene Name
IL17RA
UniProt Synonym Gene Names
IL17R; IL-17 receptor A; IL-17RA
UniProt Entry Name
I17RA_HUMAN
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Product Notes
The Human IL17RA il17ra (Catalog #AAA46142) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA46142 ELISA Kit recognizes Human IL17RA. It is sometimes possible for the material contained within the vial of "IL-17RA PicoKine, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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