Standard Curve (Sample)
Human IL-1alpha ELISA Kit | IL-1alpha elisa kit
IL-1alpha Human ELISA Kit
Gene Names
IL1A; IL1; IL-1A; IL1F1; IL1-ALPHA
Reactivity
Human
Applications
ELISA
Synonyms
IL-1alpha; N/A; IL-1alpha Human ELISA Kit; Human IL-1alpha; IL-1alpha elisa kit
Reactivity
Human
Assay Type
Sandwich
Detection Range
4.7 pg/ml-300 pg/ml
Sensitivity
< 0.5pg/ml
Applicable Applications for IL-1alpha elisa kit
ELISA
Preparation and Storage
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IL-1alpha elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-1alpha polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-1alpha polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-1alpha amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-1alpha can be calculated.
Background: Interleukin-1 alpha (IL-1alpha) is a protein of the interleukin-1 family that in humans is encoded by the IL1A gene which spans 10.2 kb and has 7 exons. It is 1 of 2 structurally distinct forms of IL1, the other being IL1B, this two proteins are synthesized by a variety of cell types, including activated macrophages, keratinocytes, stimulated B lymphocytes, and fibroblasts, and are potent mediators of inflammation and immunity. IL1A may play a role in the genesis of inflammation by augmenting the transcription of proinflammatory genes, a mechanism not affected by extracellular inhibitors. IL1A has been administered to patients during receiving autologous bone marrow transplantation.
Background: Interleukin-1 alpha (IL-1alpha) is a protein of the interleukin-1 family that in humans is encoded by the IL1A gene which spans 10.2 kb and has 7 exons. It is 1 of 2 structurally distinct forms of IL1, the other being IL1B, this two proteins are synthesized by a variety of cell types, including activated macrophages, keratinocytes, stimulated B lymphocytes, and fibroblasts, and are potent mediators of inflammation and immunity. IL1A may play a role in the genesis of inflammation by augmenting the transcription of proinflammatory genes, a mechanism not affected by extracellular inhibitors. IL1A has been administered to patients during receiving autologous bone marrow transplantation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,607 Da
NCBI Official Full Name
interleukin-1 alpha proprotein
NCBI Official Synonym Full Names
interleukin 1, alpha
NCBI Official Symbol
IL1A
NCBI Official Synonym Symbols
IL1; IL-1A; IL1F1; IL1-ALPHA
NCBI Protein Information
interleukin-1 alpha; IL-1 alpha; hematopoietin-1; preinterleukin 1 alpha; pro-interleukin-1-alpha
UniProt Protein Name
Interleukin-1 alpha
UniProt Gene Name
IL1A
UniProt Synonym Gene Names
IL1F1; IL-1 alpha
UniProt Entry Name
IL1A_HUMAN
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Product Notes
The Human IL-1alpha il1a (Catalog #AAA58922) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA58922 ELISA Kit recognizes Human IL-1alpha. AAA Biotech's IL-1alpha can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the IL-1alpha il1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-1alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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