Application Data
(This assay recognizes both recombinant and natural Human IL4. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)
Human IL4/IL-4/Interleukin-4 ELISA Kit | IL4/IL-4 elisa kit
Human IL4/IL-4/Interleukin-4 ELISA Kit
Reactivity
Human
Synonyms
IL4/IL-4/Interleukin-4; N/A; Human IL4/IL-4/Interleukin-4 ELISA Kit; IL-4 ELISA Kit, Human; interleukin 4; BCGF-1 ELISA Kit, Human; BCGF1 ELISA Kit, Human; BSF-1 ELISA Kit, Human; BSF1 ELISA Kit, Human; Interleukin-4 ELISA Kit, Human; IL4/IL-4 elisa kit
Reactivity
Human
Specificity
For the quantitative determination of Human IL4/IL-4/Interleukin-4 concentration in cell culture supernates.
Assay Type
Solid Phase Sandwich ELISA (quantitative)
Sample Types
Cell supernatant
Detection Range
10.94-700pg/mL
Sensitivity
2.54pg/mL
Conjugation
HRP
Interference
Preparations of the factors listed below at 50ng/mL in a mid-range recombinant human IL4 control were assayed for interference. No significant interference was observed.
Materials Provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Application Data
(This assay recognizes both recombinant and natural Human IL4. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)
Application Data
(This assay recognizes both recombinant and natural Human IL4. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Related Product Information for IL4/IL-4 elisa kit
This IL-4 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-4 protein in Cell supernatant. It contains recombinant Human IL-4, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Background: Interleukin-4, also known as IL4, is a secreted protein that belongs to the IL-4/IL-13 family. Interleukin-4/IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4/IL4 also regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibits in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells.
Background: Interleukin-4, also known as IL4, is a secreted protein that belongs to the IL-4/IL-13 family. Interleukin-4/IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4/IL4 also regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibits in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells.
References
Grunewald SM. et al., 1998, J Immunol. 160 (8): 4004-9.
Susanne M. et al, 1997, THE JOURNAL OF BIOLOGICAL CHEMISTRY. 272 (3): 1480-3.
Nishikubo K. et al., 2003, Gene Ther. 10 (26): 2119-25.
Susanne M. et al, 1997, THE JOURNAL OF BIOLOGICAL CHEMISTRY. 272 (3): 1480-3.
Nishikubo K. et al., 2003, Gene Ther. 10 (26): 2119-25.
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Product Notes
The Human IL4/IL-4 (Catalog #AAA258437) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA258437 ELISA Kit recognizes Human IL4/IL-4. It is sometimes possible for the material contained within the vial of "IL4/IL-4/Interleukin-4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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