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product-image-AAA120703_SC15.jpg Standard Curve (Sample)

Human IL6 ELISA Kit | IL6 elisa kit

Human IL6 ELISA Kit

Reactivity
Human
Synonyms
IL6; N/A; Human IL6 ELISA Kit; IL6 elisa kit
Ordering
Reactivity
Human
Assay Type
Quantitative Sandwich
Samples
Serum, plasma and cell culture supernatant
Detection Range
4.7 pg/mL - 300 pg/mL
Sensitivity
2.37 pg/mL
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): <10%. Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays): <15%. Three samples of known concentration were tested in twenty four separate assays to assess interassay precision.
Preparation and Storage
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% prior to the expiration date under appropriate storage condition.

Standard Curve (Sample)

product-image-AAA120703_SC15.jpg Standard Curve (Sample)
Related Product Information for IL6 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Human IL6 has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Human IL6 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled Human IL6 antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Human IL6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Intended Uses: Used for the quantitative determination of Human IL6 concentration in serum, plasma and cell culture supernatant.
Product Categories/Family for IL6 elisa kit

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Product Notes

The Human IL6 (Catalog #AAA120703) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA120703 ELISA Kit recognizes Human IL6. It is sometimes possible for the material contained within the vial of "IL6, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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