Human IP-10/CXCL10 ELISA Kit | IP-10/CXCL10 elisa kit

Human IP-10/CXCL10 ELISA Kit

Cat. Num. AAA258384

• Reactivity: Human
• Synonyms: IP-10/CXCL10; N/A; Human IP-10/CXCL10 ELISA Kit; IP-10/CXCL10 ELISA Kit, Human; chemokine (C-X-C motif) ligand 10; C7 ELISA Kit, Human; crg-2 ELISA Kit, Human; gIP-10 ELISA Kit, Human; IFI10 ELISA Kit, Human; INP10 ELISA Kit, Human; IP-10 ELISA Kit, Human; mob-1 ELISA Kit, Human; SCYB10 ELISA Kit, Human; IP-10/CXCL10 elisa kit

• Reactivity: Human
• Specificity: This assay recognizes both recombinant and natural Human CXCL10. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
• Assay Type: Quantitative Sandwich
• Samples: Serum
• Sensitivity: < 2.58 pg/mL

• Intra-assay Precision: Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision: Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Standard Curve (Sample)

Related Product Information for IP-10/CXCL10 elisa kit

Background: (C-X-C motif) ligand (CXCL)10 (CXCL10) belongs to the ELR(-) CXC subfamily chemokine. CXCL10/IP-10 exerts its function through binding to chemokine (C-X-C motif) receptor 3 (CXCR3), a seven trans-membrane receptor coupled to G proteins. CXCL10/IP-10 and its receptor, CXCR3, appear to contribute to the pathogenesis of many autoimmune diseases, organ specific (such as type 1 diabetes, autoimmune thyroiditis, Graves' disease and ophthalmopathy), or systemic (such as rheumatoid arthritis, psoriatic arthritis, systemic lupus erythematosus, mixed cryoglobulinemia, Sjögren syndrome, or systemic sclerosis). CXCL10/IP-10 is secreted by several cell types including endothelial cells, fibroblasts, keratinocytes, thyrocytes, preadipocytes, etc. Determination of high level of CXCL10/IP-10 in peripheral fluids is therefore a marker of host immune response.

Principle of the Assay: The principle of this ELISA kit is based on the solid phase sandwich enzyme immunoassay technique. A monoclonal antibody specific for Human IP-10/CXCL10 has been pre-coated onto well plate strips. Standards and samples are added to the wells and Human IP-10/CXCL10 present in the sample is bound by the immobilized antibody. After incubation the wells are washed and a horseradish peroxidase conjugated anti-Human IP-10/CXCL10 antibody is added, producing an antibody-antigen-antibody "sandwich complex". Following a wash to remove any unbound antibody a TMB substrate solution is loaded and color develops in proportion to the amount of Human IP-10/CXCL10 bound. The reaction is stopped by the addition of a stop solution and the intensity of the color can be measured at 450 nm (See schematics below).

Intended Uses: For the quantitative determination of Human IP-10/CXCL10 concentration in serum.

References

O'Donovan N, et al. (1999) Physical mapping of the CXC chemokine locus on human chromosome 4. Cytogenet. Cell Genet. 84(1-2):39-42.
Swaminathan GJ, et al. (2003) Crystal structures of oligomeric forms of the IP-10/CXCL10 chemokine. Structure. 11(5):521-32.
Booth V, et al. (2002) The CXCR3 binding chemokine IP-10/CXCL10: structure and receptor interactions. Biochemistry. 41(33):10418-25.