Mouse CXCL10/IP-10 ELISA Kit | CXCL10/IP-10 elisa kit
Mouse CXCL10/IP-10 PicoKine Quick ELISA Kit
Gene Names
Cxcl10; C7; IP10; CRG-2; INP10; IP-10; Ifi10; mob-1; Scyb10; gIP-10
Reactivity
Mouse
Applications
ELISA
Synonyms
CXCL10/IP-10; N/A; Mouse CXCL10/IP-10 PicoKine Quick ELISA Kit; CXCL10/IP-10 elisa kit
Reactivity
Mouse
Samples
cell culture supernatants, serum and plasma(heparin)
Assay Range
31.2pg/ml-2,000pg/ml
Sensitivity
<1pg/ml
Capture antibody and Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat
Immunogen
Expression system for standard: E coli; Immunogen sequence: I22-P98
Anticoagulant
heparin
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Applicable Applications for CXCL10/IP-10 elisa kit
ELISA
Kit Components
Description/Quantity/Volume
Anti-tag Pre-coated 96-well Strip Microplate/1/12 strips of 8 wells
Standard/2, 10 ng/tube
Antibody A/1/3ml
Antibody B/1/3ml
Sample Diluent/1/15ml
TBS-T Wash Buffer (25x)/1/12ml
Color Developing Reagent (TMB)/1/10ml
Stop Solution/1/10ml
Adhesive plate sealers/2/Piece
Anti-tag Pre-coated 96-well Strip Microplate/1/12 strips of 8 wells
Standard/2, 10 ng/tube
Antibody A/1/3ml
Antibody B/1/3ml
Sample Diluent/1/15ml
TBS-T Wash Buffer (25x)/1/12ml
Color Developing Reagent (TMB)/1/10ml
Stop Solution/1/10ml
Adhesive plate sealers/2/Piece
Materials Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer (optional)
Pipettes and pipette tips capable of precisely dispensing 0.5ul through 1ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500rpm, amplitude 3mm.
Automated plate washer (optional)
Pipettes and pipette tips capable of precisely dispensing 0.5ul through 1ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500rpm, amplitude 3mm.
Sample Data
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.036 | 0.080 | 0.110 | 0.178 | 0.328 | 0.619 | 1.151 | 1.956 |
Intra Assay Consistency & Inter Assay Consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 105 | 245 | 923 | 110 | 248 | 879 |
| Standard deviation | 10.237 | 13.72 | 47.99 | 10.23 | 18.6 | 56.25 |
| CV (%) | 7.5% | 5.6% | 5.2% | 9.3% | 7.5% | 6.4% |
Reproducibility
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 105 | 98 | 104 | 102 | 102 | 2.68 | 2.6% |
| Sample 2 | 245 | 274 | 275 | 259 | 263 | 12.29 | 4.6% |
| Sample 3 | 923 | 908 | 845 | 862 | 884 | 32.01 | 3.6% |
Preparation and Storage
Store at 4 degree C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Related Product Information for CXCL10/IP-10 elisa kit
The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Product Categories/Family for CXCL10/IP-10 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
10,789 Da
NCBI Official Full Name
IP-10
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 10
NCBI Official Symbol
Cxcl10
NCBI Official Synonym Symbols
C7; IP10; CRG-2; INP10; IP-10; Ifi10; mob-1; Scyb10; gIP-10
NCBI Protein Information
C-X-C motif chemokine 10; gamma-IP10; interferon activated gene 10; small-inducible cytokine B10; interferon-gamma induced protein CRG-2; interferon-gamma-induced protein CRG-2; 10 kDa interferon gamma-induced protein; small inducible cytokine B subfamily
UniProt Protein Name
C-X-C motif chemokine 10
UniProt Gene Name
Cxcl10
UniProt Synonym Gene Names
Crg2; Ifi10; Inp10; Scyb10; Gamma-IP10; IP-10
UniProt Entry Name
CXL10_MOUSE
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Product Notes
The Mouse CXCL10/IP-10 cxcl10 (Catalog #AAA128071) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA128071 ELISA Kit recognizes Mouse CXCL10/IP-10. AAA Biotech's CXCL10/IP-10 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the CXCL10/IP-10 cxcl10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CXCL10/IP-10, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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