Human Laminin Gamma 1 (LAMC1) ELISA Kit | LAMC1 elisa kit

Human Laminin Gamma 1 (LAMC1) ELISA Kit

Cat. Num. AAA13968

• Gene Names: LAMC1; LAMB2
• Reactivity: Human
• Synonyms: Laminin Gamma 1 (LAMC1); N/A; Human Laminin Gamma 1 (LAMC1) ELISA Kit; LAM-C1; LAMB2; S-laminin subunit gamma; Laminin B2 chain; LAMC1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of LAMC1. No significant cross-reactivity or interference between LAMC1 and analogues was observed.
• Sequence Length: 1609
• Assay Type: Quantitative Sandwich
• Samples: Human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids
• Detection Range: 31.2-2000pg/mL
• Sensitivity: < 12.1pg/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LAMC1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LAMC1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Standard Curve (Sample)

Related Product Information for LAMC1 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LAMC1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LAMC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LAMC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LAMC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of LAMC1 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

NCBI and Uniprot Product Information

• NCBI GI #: 145309326
• NCBI GeneID: 3915
• NCBI Accession #: NP_002284.3
• NCBI GenBank Nucleotide #: NM_002293.3
• UniProt Accession #: P11047; Q5VYE7
• Molecular Weight: 177,603 Da
• NCBI Official Full Name: laminin subunit gamma-1
• NCBI Official Synonym Full Names: laminin subunit gamma 1
• NCBI Official Symbol: LAMC1
• NCBI Official Synonym Symbols: LAMB2
• NCBI Protein Information: laminin subunit gamma-1
• UniProt Protein Name: Laminin subunit gamma-1
• UniProt Gene Name: LAMC1
• UniProt Synonym Gene Names: LAMB2; S-LAM gamma