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Application Data
(Figure 4 - Detection of laminin in human kidney frozen tissue section using monoclonal Rat anti Laminin diluted 1:100)
Rat Laminin (LAMC1) Monoclonal Antibody | anti-LAMC1 antibody
Laminin (LAMC1), Monoclonal Antibody
Gene Names
LAMC2; B2T; CSF; EBR2; BM600; EBR2A; LAMB2T; LAMNB2; MGC138491; MGC141938
Reactivity
Human, Mouse, Swine.
Applications
Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Synonyms
Laminin (LAMC1), Antibody; Laminin (LAMC1), Monoclonal Antibody; anti-LAMC1 antibody
Host
Rat
Reactivity
Human, Mouse, Swine.
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
A5
Specificity
A5 reacts with laminin.
Form/Format
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-LAMC1 antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry)
Source Note
A5 is a Rat monoclonal IgG2a antibody derived by fusion of Mouse myeloma cells with spleen cells from a Fisher Rat immunized with a laminin prepaRation from the EHS Mouse tumor.
Preparation and Storage
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Application Data
(Figure 4 - Detection of laminin in human kidney frozen tissue section using monoclonal Rat anti Laminin diluted 1:100)
Application Data
(Figure 4 - Detection of laminin in human kidney frozen tissue section using monoclonal Rat anti Laminin diluted 1:100)
IHC (Immunohistochemisry)
(Figure 3 - Detection of laminin in human kidney frozen tissue section using monoclonal Rat anti Laminin diluted 1:100)
IHC (Immunohistochemisry)
(Figure 3 - Detection of laminin in human kidney frozen tissue section using monoclonal Rat anti Laminin diluted 1:100)
IF (Immunofluorescence)
(Figure 2. Immunofluorescence staining of frozen sections from pig striated skeletal muscle (methanol fixed) showing the localization of laminin in the connective tissue and in basement membranes surrounding the myofibrils.)
IF (Immunofluorescence)
(Figure 2. Immunofluorescence staining of frozen sections from pig striated skeletal muscle (methanol fixed) showing the localization of laminin in the connective tissue and in basement membranes surrounding the myofibrils.)
IF (Immunofluorescence)
(Figure 1. Immunofluorescence staining of frozen sections from human colon (methanol fixed), showing the localization of laminin in the connective tissue and in basement membranes surrounding the epithelial crypts and villi.)
IF (Immunofluorescence)
(Figure 1. Immunofluorescence staining of frozen sections from human colon (methanol fixed), showing the localization of laminin in the connective tissue and in basement membranes surrounding the epithelial crypts and villi.)
Related Product Information for anti-LAMC1 antibody
Basement membrane components play an important role in cell adhesion, locomotion, differentiation and embryonic compartmentalization. Laminin anchors cells to the basement membrane through interactions with integrins at the cell surface and collagens in the basement membrane.
Product Categories/Family for anti-LAMC1 antibody
References
Ljubimov, A. V., Bartek, J., Couchman, J. R., Kapuller, L. L., Veselov, V. V., Kovarik, J., Perevoshchikov, A. G., and Krutovskikh, V. A. (1992). Distribution of individual components of basement membrane in Human colon polyps and adenocarcinomas as revealed by monoclonal antibodies. Int J Cancer 50, 562-66. Ljubimova, J.Y., Lakhter, A.J., Loksh, A., Yong, W.H., Riedinger, M.S., Miner, J.H., Sorokin, L.M., Ljubimov, A.V., Black, K.L. (2001). Overexpression of alpha4, chain-containing laminins in Human glial tumors identified by gene microarray analysis. Cancer Research 61, 5601-10.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Laminin
NCBI Official Synonym Full Names
laminin, gamma 2
NCBI Official Symbol
LAMC2
NCBI Official Synonym Symbols
B2T; CSF; EBR2; BM600; EBR2A; LAMB2T; LAMNB2; MGC138491; MGC141938
NCBI Protein Information
laminin subunit gamma-2; BM600-100kDa; nicein-100kDa; kalinin-105kDa; kalinin (105kD); ladsin (140kDa); nicein (100kDa); laminin B2t chain; OTTHUMP00000033550; OTTHUMP00000230852; CSF 140 kDa subunit; nicein subunit gamma; kalinin subunit gamma; ladsin 140 kDa subunit; epiligrin subunit gamma; laminin-5 subunit gamma; cell-scattering factor 140 kDa subunit; large adhesive scatter factor 140 kDa subunit
UniProt Protein Name
Laminin, gamma 2
UniProt Gene Name
LAMC2
UniProt Entry Name
Q2M1N2_HUMAN
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Product Notes
The LAMC1 lamc2 (Catalog #AAA77479) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Laminin (LAMC1), Monoclonal Antibody reacts with Human, Mouse, Swine. and may cross-react with other species as described in the data sheet. AAA Biotech's Laminin (LAMC1) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the LAMC1 lamc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Laminin (LAMC1), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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