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product-image-AAA46000_SC15.jpg Standard Curve (Sample) (Human LIFR PicoKine ELISA Kit standard curve)

Human LIFR ELISA Kit | LIFR elisa kit

Human LIFR PicoKine ELISA Kit

Gene Names
LIFR; SWS; SJS2; STWS; CD118; LIF-R
Reactivity
Human
Synonyms
LIFR; N/A; Human LIFR PicoKine ELISA Kit; Leukemia inhibitory factor receptor; leukemia inhibitory factor receptor alpha; CD118; CD118 antigen; FLJ98106; FLJ99923; Leukemia inhibitory factor receptor alpha; LIF R; LIF receptor; LIF-R; Lifr; LIFR_HUMAN; SJS2; STWS; SWS; LIFR elisa kit
Ordering
Reactivity
Human
Specificity
Natural and recombinant human LIFR
Sequence Length
1097
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Human LIFR PicoKine ELISA Kit standard curve)

product-image-AAA46000_SC15.jpg Standard Curve (Sample) (Human LIFR PicoKine ELISA Kit standard curve)
Related Product Information for LIFR elisa kit
Principle of the assay: human LIFR ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for LIFR has been precoated onto 96-well plates. Standards(NSO, Q45-S833) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LIFR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human LIFR amount of sample captured in plate.
Background: LIFR, also known as CD118 (Cluster of Differentiation 118), is a subunit of a receptor for leukemia inhibitory factor. This gene encodes a protein that belongs to the type I cytokine receptor family. It is mapped to 5p31.1. The LIF receptor (LIFR) is the low-affinity binding chain that, together with the high-affinity converter subunit gp130, forms a high-affinity receptor complex that mediates the action of the leukemia-inhibitory factor. LIF is a polyfunctional cytokine that affects the differentiation, survival, and proliferation of a wide variety of cells in the adult and the embryo. Mutations in this gene cause Schwartz-Jampel syndrome type 2, a disease belonging to the group of the bent-bone dysplasias. A translocation that involves the promoter of this gene, togerther with the pleiomorphic adenoma gene 1, is associated with salivary gland pleiomorphic adenoma, a common type of benign epithelial tumor of the salivary gland.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
– Da
NCBI Official Full Name
Leukemia inhibitory factor receptor
NCBI Official Synonym Full Names
leukemia inhibitory factor receptor alpha
NCBI Official Symbol
LIFR
NCBI Official Synonym Symbols
SWS; SJS2; STWS; CD118; LIF-R
NCBI Protein Information
leukemia inhibitory factor receptor; LIF receptor; CD118 antigen
UniProt Protein Name
Leukemia inhibitory factor receptor
UniProt Gene Name
LIFR
UniProt Synonym Gene Names
LIF receptor; LIF-R
UniProt Entry Name
LIFR_HUMAN

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Product Notes

The Human LIFR lifr (Catalog #AAA46000) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA46000 ELISA Kit recognizes Human LIFR. It is sometimes possible for the material contained within the vial of "LIFR, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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