Human LILRB1 ELISA Kit | LILRB1 elisa kit

Human LILRB1 ELISA Kit

Cat. Num. AAA125843

• Gene Names: LILRB1; ILT2; LIR1; MIR7; CD85J; ILT-2; LIR-1; MIR-7
• Reactivity: Human
• Synonyms: LILRB1; N/A; Human LILRB1 ELISA Kit; Leukocyte immunoglobulin-like receptor subfamily B member 1; LIR-1; Leukocyte immunoglobulin-like receptor 1; CD85 antigen-like family member J; Immunoglobulin-like transcript 2; ILT-2; Monocyte/macrophage immunoglobulin-like receptor 7; MIR-7; CD85j; ILT2; LIR1; MIR7; leukocyte immunoglobulin like receptor B1; LILRB1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human LILRB1
• Samples: cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
• Detection Range: 312pg/ml-20,000pg/ml
• Sensitivity: <50pg/ml
• Capture/Detection Antibodies: Capture antibody- a monoclonal antibody from mouse|Detection antibody- a biotinylated polyclonal antibody from goat
• Sample Data: Concentration(pg/ml)|0|312|625|1250|2500|5000|10000|20000; O.D.|0.062|0.144|0.218|0.378|0.612|0.939|1.446|2.059
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|364|2379|7370|; Standard deviation|18.93|126.09|405.35|; CV(%)|5.2%|5.3%|5.5%|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|417|2441|8736; Standard deviation|25.44|156.22|585.31; CV(%)|6.1%|6.4%|6.7%

• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 364|349|378|353|361|11.24|3.1%; Sample 2: 2379|2185|2390|2124|2269|117.06|5.1%; Sample 3: 7370|7573|8346|7810|7774|364.72|4.6%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: G24-V461
• Anticoagulant: heparin, EDTA or citrate
• Principle: Human LILRB1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for LILRB1 has been precoated onto 96-well plates. Standards(Expression system for standard: NS0, Immunogen sequence: G24-V461) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LILRB1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human LILRB1 amount of sample captured in plate.
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 20000pg/ml, 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml human LILRB1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatants, serum or plasma (heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" for details. We recommended that each human LILRB1 standard solution and each sample be measured in duplicate.
• TMB Incubation Time: 15-20
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Standard Curve (Sample)

(Human LILRB1 ELISA Kit Standard Curve)

Related Product Information for LILRB1 elisa kit

Leukocyte immunoglobulin-like receptors (LILRs), also called CD85s, ILTs, or LIRs, are important mediators of immune activation and tolerance that contain tandem immunoglobulin (Ig)-like folds. LILRB1 is encoded within the leukocyte receptor complex on 19q13. 4, previously implicated to be a susceptibility region to systemic lupus erythematosus (SLE). LILRB1 binds to a wide range of major histocompatibility complex class I molecules (MHCIs) and transduces negative signals that can, for example, prevent killing of MHCI-expressing cells. LILRB1 transcription is correlated with surface protein expression on NK cells.

Product Categories/Family for LILRB1 elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GI #: 612149795
• NCBI GeneID: 10859
• NCBI Accession #: NP_001075108.2
• NCBI GenBank Nucleotide #: NM_001081639.3
• UniProt Accession #: Q8NHL6; O75024; O75025; Q8NHJ9; Q8NHK0; A2IXV4; A8MXT0
• Molecular Weight: 70,819 Da
• NCBI Official Full Name: leukocyte immunoglobulin-like receptor subfamily B member 1 isoform 4
• NCBI Official Synonym Full Names: leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1
• NCBI Official Symbol: LILRB1
• NCBI Official Synonym Symbols: ILT2; LIR1; MIR7; CD85J; ILT-2; LIR-1; MIR-7
• NCBI Protein Information: leukocyte immunoglobulin-like receptor subfamily B member 1; Ig-like transcript 2; CD85 antigen-like family member J; monocyte/macrophage immunoglobulin-like receptor 7; leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform
• UniProt Protein Name: Leukocyte immunoglobulin-like receptor subfamily B member 1
• UniProt Gene Name: LILRB1
• UniProt Synonym Gene Names: ILT2; LIR1; MIR7; LIR-1; Leukocyte immunoglobulin-like receptor 1; ILT-2; MIR-7
• UniProt Entry Name: LIRB1_HUMAN