Standard Curve (Sample)
(Fig.1. Human Long-chain-fatty-acid--CoA ligase 1 (ACSL1) Standard Curve.)
Human Long-Chain-Fatty-Acid--CoA Ligase 1 (ACSL1) ELISA Kit | ACSL1 elisa kit
Human Long-Chain-Fatty-Acid--CoA Ligase 1 (ACSL1) ELISA Kit
Gene Names
ACSL1; ACS1; LACS; FACL1; FACL2; LACS1; LACS2
Reactivity
Human
Synonyms
Long-Chain-Fatty-Acid--CoA Ligase 1 (ACSL1); N/A; Human Long-Chain-Fatty-Acid--CoA Ligase 1 (ACSL1) ELISA Kit; ACSL1; ACS1; FACL1; FACL2; LACS; LACS1; LACS2; fatty-acid-Coenzyme A ligase; long-chain 1; long-chain 2; lignoceroyl-CoA synthase; long-chain acyl-CoA synthetase 1; long-chain acy; ACSL1 elisa kit
Reactivity
Human
Specificity
Human Long-chain-fatty-acid--CoA ligase 1 (ACSL1) ELISA Kit has high sensitivity and excellent specificity for detection of Human ACSL1. No significant cross-reactivity or interference between Human ACSL1 and analogues was observed.
Sequence Length
698
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Long-chain-fatty-acid--CoA ligase 1 (ACSL1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Long-chain-fatty-acid--CoA ligase 1 (ACSL1) Standard Curve.)
Related Product Information for ACSL1 elisa kit
Description: This Human Long-chain-fatty-acid--CoA ligase 1 (ACSL1) ELISA Kit employs a two-site sandwich ELISA to quantitate ACSL1 in samples. An antibody specific for ACSL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACSL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACSL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACSL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Long-chain-fatty-acid--CoA ligase 1 is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. In melanocytic cells ACSL1 gene expression may be regulated by MITF.Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate. Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas.
Background: Long-chain-fatty-acid--CoA ligase 1 is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. In melanocytic cells ACSL1 gene expression may be regulated by MITF.Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate. Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Long-chain-fatty-acid--CoA ligase 1
NCBI Official Synonym Full Names
acyl-CoA synthetase long chain family member 1
NCBI Official Symbol
ACSL1
NCBI Official Synonym Symbols
ACS1; LACS; FACL1; FACL2; LACS1; LACS2
NCBI Protein Information
long-chain-fatty-acid--CoA ligase 1
UniProt Protein Name
Long-chain-fatty-acid--CoA ligase 1
UniProt Gene Name
ACSL1
UniProt Synonym Gene Names
FACL1; FACL2; LACS; LACS1; LACS2; ACS1; LACS 1; LACS 2
UniProt Entry Name
ACSL1_HUMAN
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Product Notes
The Human ACSL1 acsl1 (Catalog #AAA331078) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331078 ELISA Kit recognizes Human ACSL1. It is sometimes possible for the material contained within the vial of "Long-Chain-Fatty-Acid--CoA Ligase 1 (ACSL1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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