Standard Curve (Sample)
Human Lysophospholipase III (LYPLA3) ELISA Kit | LYPLA3 elisa kit
Human Lysophospholipase III (LYPLA3)(Sandwich ELISA) ELISA Kit
Gene Names
PLA2G15; ACS; LLPL; LPLA2; LYPLA3; GXVPLA2
Reactivity
Human
Synonyms
Lysophospholipase III (LYPLA3); N/A; Human Lysophospholipase III (LYPLA3)(Sandwich ELISA) ELISA Kit; ELISA Kit for Lysophospholipase III (LYPLA3); PLA2G15; ACS; LLPL; LPLA2; GXVPLA2; Lysosomal Phospholipase A2; Group XV Phospholipase A2; 1-O-acylceramide synthase; LCAT-like lysophospholipase; LYPLA3 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of LYPLA3. No significant cross-reactivity or interference between LYPLA3 and analogues was observed.
Assay Type
Quantitative Sandwich
Samples
Human serum, plasma, tissue homogenatesand other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.055ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LYPLA3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LYPLA3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 4 degree C for 1 month/-20 degree C for long-term (within shelf life)
Shelf-life: one year
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Shelf-life: one year
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for LYPLA3 elisa kit
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to LYPLA3. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to LYPLA3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LYPLA3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LYPLA3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LYPLA3 in human serum, plasma, tissue homogenatesand other biological fluids.
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LYPLA3 in human serum, plasma, tissue homogenatesand other biological fluids.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
35,717 Da
NCBI Official Full Name
lysophospholipase 3 (lysosomal phospholipase A2), isoform CRA_b, partial
NCBI Official Synonym Full Names
phospholipase A2 group XV
NCBI Official Symbol
PLA2G15
NCBI Official Synonym Symbols
ACS; LLPL; LPLA2; LYPLA3; GXVPLA2
NCBI Protein Information
group XV phospholipase A2
UniProt Protein Name
Group XV phospholipase A2
UniProt Gene Name
PLA2G15
UniProt Synonym Gene Names
LYPLA3; ACS; LLPL1 PublicationManual assertion based on opinion iniRef.1; LPLA22 PublicationsManual assertion based on opinion iniRef.11
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Product Notes
The Human LYPLA3 pla2g15 (Catalog #AAA152367) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA152367 ELISA Kit recognizes Human LYPLA3. It is sometimes possible for the material contained within the vial of "Lysophospholipase III (LYPLA3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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