Standard Curve (Sample)
(Fig.1. Human Mammalian target of rapamyein (MTOR) Standard Curve.)
Human Mammalian Target Of Rapamyein (MTOR) ELISA Kit | MTOR elisa kit
Human Mammalian Target Of Rapamyein (MTOR) ELISA Kit
Reactivity
Human
Synonyms
Mammalian Target Of Rapamyein (MTOR); N/A; Human Mammalian Target Of Rapamyein (MTOR) ELISA Kit; MTOR elisa kit
Reactivity
Human
Specificity
Human Mammalian target of rapamyein (MTOR) ELISA Kit has high sensitivity and excellent specificity for detection of Human MTOR. No significant cross-reactivity or interference between Human MTOR and analogues was observed.
Sequence Length
2549
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Mammalian target of rapamyein (MTOR) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Mammalian target of rapamyein (MTOR) Standard Curve.)
Related Product Information for MTOR elisa kit
Description: This Human Mammalian target of rapamyein (MTOR) ELISA Kit employs a two-site sandwich ELISA to quantitate MTOR in samples. An antibody specific for MTOR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MTOR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTOR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTOR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Mammalian target of rapamyein encoded by MTOR belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of MTOR. MTOR (Mechanistic Target Of Rapamycin) is a Protein Coding gene. Diseases associated with MTOR include Smith-Kingsmore Syndrome and Macrocephaly-Intellectual Disability-Neurodevelopmental Disorder-Small Thorax Syndrome. Among its related pathways are CXCR4-mediated signaling events and RET signaling. GO annotations related to MTOR include transferase activity, transferring phosphorus-containing groups and protein serine/threonine kinase activity. An important paralog of this gene is SMG1.
Background: Mammalian target of rapamyein encoded by MTOR belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of MTOR. MTOR (Mechanistic Target Of Rapamycin) is a Protein Coding gene. Diseases associated with MTOR include Smith-Kingsmore Syndrome and Macrocephaly-Intellectual Disability-Neurodevelopmental Disorder-Small Thorax Syndrome. Among its related pathways are CXCR4-mediated signaling events and RET signaling. GO annotations related to MTOR include transferase activity, transferring phosphorus-containing groups and protein serine/threonine kinase activity. An important paralog of this gene is SMG1.
NCBI and Uniprot Product Information
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Product Notes
The Human MTOR (Catalog #AAA331642) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331642 ELISA Kit recognizes Human MTOR. It is sometimes possible for the material contained within the vial of "Mammalian Target Of Rapamyein (MTOR), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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