Standard Curve (Sample)
(Fig.1. Human Matrix metallopeptidase 25 (MMP25) Standard Curve.)
Human Matrix Metallopeptidase 25 (MMP25) ELISA Kit | MMP25 elisa kit
Human Matrix Metallopeptidase 25 (MMP25) ELISA Kit
Gene Names
MMP25; MMP20; MMPL1; MMP-25; MMP20A; MT6MMP; MTMMP6; MT-MMP6; MT6-MMP; MT-MMP 6
Reactivity
Human
Synonyms
Matrix Metallopeptidase 25 (MMP25); N/A; Human Matrix Metallopeptidase 25 (MMP25) ELISA Kit; MMP25; MMP20; MMP20A; MMPL1; MT-MMP6; MT6-MMP; leukolysin; matrix metallopeptidase-like 1; matrix metalloproteinase 20; matrix metalloproteinase 25; matrix metalloproteinase-like 1; membrane-type 6 matrix metal; MMP25 elisa kit
Reactivity
Human
Specificity
Human Matrix metallopeptidase 25 (MMP25) ELISA Kit has high sensitivity and excellent specificity for detection of Human MMP25. No significant cross-reactivity or interference between Human MMP25 and analogues was observed.
Sequence Length
562
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Matrix metallopeptidase 25 (MMP25) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Matrix metallopeptidase 25 (MMP25) Standard Curve.)
Related Product Information for MMP25 elisa kit
Description: This Human Matrix metallopeptidase 25 (MMP25) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP25 in samples. An antibody specific for MMP25 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP25 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP25 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP25 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily, attached to the plasma membrane via a glycosylphosphatidyl inositol anchor. In response to bacterial infection or inflammation, the encoded protein is thought to inactivate alpha-1 proteinase inhibitor, a major tissue protectant against proteolytic enzymes released by activated neutrophils, facilitating the transendothelial migration of neutrophils to inflammatory sites. The encoded protein may also play a role in tumor invasion and metastasis through activation of MMP2. The gene has previously been referred to as MMP20 but has been renamed MMP25.
Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily, attached to the plasma membrane via a glycosylphosphatidyl inositol anchor. In response to bacterial infection or inflammation, the encoded protein is thought to inactivate alpha-1 proteinase inhibitor, a major tissue protectant against proteolytic enzymes released by activated neutrophils, facilitating the transendothelial migration of neutrophils to inflammatory sites. The encoded protein may also play a role in tumor invasion and metastasis through activation of MMP2. The gene has previously been referred to as MMP20 but has been renamed MMP25.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Matrix metalloproteinase-25
NCBI Official Synonym Full Names
matrix metallopeptidase 25
NCBI Official Symbol
MMP25
NCBI Official Synonym Symbols
MMP20; MMPL1; MMP-25; MMP20A; MT6MMP; MTMMP6; MT-MMP6; MT6-MMP; MT-MMP 6
NCBI Protein Information
matrix metalloproteinase-25
UniProt Protein Name
Matrix metalloproteinase-25
UniProt Gene Name
MMP25
UniProt Synonym Gene Names
MMP20; MMPL1; MT6MMP; MMP-25; MT-MMP 6; MTMMP6; MT6-MMP; MT6MMP
UniProt Entry Name
MMP25_HUMAN
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Product Notes
The Human MMP25 mmp25 (Catalog #AAA331745) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331745 ELISA Kit recognizes Human MMP25. It is sometimes possible for the material contained within the vial of "Matrix Metallopeptidase 25 (MMP25), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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