WB (Western Blot)
(All lanes : Anti-MMP25 Antibody (R485) at 1:1000 dilutionLane 1: HT-29 whole cell lysateLane 2: HL-60 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 63 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human MMP25 Polyclonal Antibody | anti-MMP25 antibody
MMP25 Antibody (C-term)
Gene Names
MMP25; MMP20; MMPL1; MMP-25; MMP20A; MT6MMP; MTMMP6; MT-MMP6; MT6-MMP; MT-MMP 6
Reactivity
Human
Applications
ELISA, Western Blot
Synonyms
MMP25, Antibody; MMP25 Antibody (C-term); Matrix metalloproteinase-25; MMP-25; 3424-; Leukolysin; Membrane-type matrix metalloproteinase 6; MT-MMP 6; MTMMP6; Membrane-type-6 matrix metalloproteinase; MT6-MMP; MT6MMP; MMP25; MMP20; MMPL1; anti-MMP25 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This MMP25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 470-499 amino acids from the C-terminal region of human MMP25.
Sequence Length
562
Applicable Applications for anti-MMP25 antibody
ELISA, WB (Western Blot)
Antigen Source
Human
Antigen Region
470-499
Antigen Type
Recombinant Protein
Function
May activate progelatinase A.
Cellular Location
Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. Secreted, extracellular space, extracellular matrix
Tissue Location
Expressed predominantly in leukocytes, lung and spleen. Expressed also in colon carcinoma, astrocytoma and glioblastomas
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(All lanes : Anti-MMP25 Antibody (R485) at 1:1000 dilutionLane 1: HT-29 whole cell lysateLane 2: HL-60 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 63 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-MMP25 Antibody (R485) at 1:1000 dilutionLane 1: HT-29 whole cell lysateLane 2: HL-60 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 63 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-MMP25 antibody
Description: Purified Rabbit Polyclonal Antibody (Pab)
Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, MMP25 is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. In addition, it is thought that this protein is activated intracellularly by furin-like enzymes. This protein activates MMP2 by cleavage. The gene has previously been referred to as MMP20 but has been renamed matrix metalloproteinase 25 (MMP25).
Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, MMP25 is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. In addition, it is thought that this protein is activated intracellularly by furin-like enzymes. This protein activates MMP2 by cleavage. The gene has previously been referred to as MMP20 but has been renamed matrix metalloproteinase 25 (MMP25).
Product Categories/Family for anti-MMP25 antibody
References
Matsuda, A., et al., J. Biol. Chem. 278(38):36350-36357 (2003). Velasco, G., et al., Cancer Res. 60(4):877-882 (2000). Nagase, H., et al., J. Biol. Chem. 274(31):21491-21494 (1999). Pei, D., Cell Res. 9(4):291-303 (1999). Kojima, S., et al., FEBS Lett. 480 (2-3), 142-146 (2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Calculated MW: H=63 kDa
NCBI Official Full Name
Matrix metalloproteinase-25
NCBI Official Synonym Full Names
matrix metallopeptidase 25
NCBI Official Symbol
MMP25
NCBI Official Synonym Symbols
MMP20; MMPL1; MMP-25; MMP20A; MT6MMP; MTMMP6; MT-MMP6; MT6-MMP; MT-MMP 6
NCBI Protein Information
matrix metalloproteinase-25
UniProt Protein Name
Matrix metalloproteinase-25
UniProt Gene Name
MMP25
UniProt Synonym Gene Names
MMP20; MMPL1; MT6MMP; MMP-25; MT-MMP 6; MTMMP6; MT6-MMP; MT6MMP
UniProt Entry Name
MMP25_HUMAN
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Product Notes
The MMP25 mmp25 (Catalog #AAA290953) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MMP25 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MMP25 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MMP25 mmp25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MMP25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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