Human MEGF9 ELISA Kit | MEGF9 elisa kit

Human MEGF9 ELISA Kit PicoKine

Cat. Num. AAA128029

• Gene Names: MEGF9; EGFL5
• Reactivity: Human
• Applications: ELISA
• Synonyms: MEGF9; N/A; Human MEGF9 ELISA Kit PicoKine; MEGF9 elisa kit

• Reactivity: Human
• Samples: cell culture supernatants, serum and plasma (heparin, EDTA)
• Assay Range: 156pg/ml-10,000pg/ml
• Sensitivity: <75pg/ml
• Capture antibody and Detection antibody: polyclonal antibody from goat|polyclonal antibody from goat
• Immunogen: Expression system for standard: NS0; Immunogen sequence: A36-N514
• Anticoagulant: heparin or EDTA
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

• Applicable Applications for MEGF9 elisa kit: ELISA
• Kit Components: Description/Quantity; Pre-coated 96-well strip microplate/1; Standard/2 vials, 10 ng/tube; Biotinylated antibody (100x)/100ul; Avidin-Biotin-Peroxidase Complex (100x)/100ul; Sample Diluent/30ml; Antibody Diluent/12ml; Avidin-Biotin-Peroxidase Diluent/12ml; Color Developing Reagent (TMB)/10ml; Stop Solution/10ml; Wash Buffer (25x)/20ml; Adhesive plate sealers/4
• Materials Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm.; Incubator.; Automated plate washer (optional).; Pipettes and pipette tips capable of precisely dispensing 0.5ul through 1ml volumes of aqueous solutions.; Multichannel pipettes are recommended for large amount of samples.; Deionized or distilled water.; 500ml graduated cylinders.; Test tubes for dilution.
• Sample Data:

  Concentration(pg/ml)	0	156	312	625	1,250	2,500	5,000	10,000
  O.D.	0.046	0.161	0.258	0.432	0.663	0.987	1.361	2.056

• Intra Assay Consistency & Inter Assay Consistency:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	223	315	3772	266	463	4194
  Standard deviation	12.93	19.47	117.31	5.61	36.3	249.54
  CV(%)	5.8%	6.2%	3.1%	2.1%	7.8%	6.0%

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	223	215	175	306	230	16.96	7.4%
  Sample 2	315	457	345	413	383	26.12	6.8%
  Sample 3	3772	4291	2918	4826	3952	199.17	5%

  *number of samples for each test n=16.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Standard Curve (Sample)

(Human MEGF9 PicoKine ELISA Kit standard curve)

Related Product Information for MEGF9 elisa kit

The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Categories/Family for MEGF9 elisa kit

PicoKine ELISA Kits

NCBI and Uniprot Product Information

• NCBI GI #: 304434526
• NCBI GeneID: 1955
• NCBI Accession #: NP_001073966.2
• NCBI GenBank Nucleotide #: NM_001080497.2
• UniProt Accession #: Q9H1U4; O75098; B7Z315
• Molecular Weight: 62,984 Da
• NCBI Official Full Name: multiple epidermal growth factor-like domains protein 9
• NCBI Official Synonym Full Names: multiple EGF-like-domains 9
• NCBI Official Symbol: MEGF9
• NCBI Official Synonym Symbols: EGFL5
• NCBI Protein Information: multiple epidermal growth factor-like domains protein 9; EGF-like-domain, multiple 5; epidermal growth factor-like protein 5
• UniProt Protein Name: Multiple epidermal growth factor-like domains protein 9
• UniProt Gene Name: MEGF9
• UniProt Synonym Gene Names: EGFL5; KIAA0818; Multiple EGF-like domains protein 9
• UniProt Entry Name: MEGF9_HUMAN