Standard Curve (Sample)
Human Metallothionein-1G (MT1G) ELISA Kit | MT1G elisa kit
Human Metallothionein-1G (MT1G) ELISA Kit
Gene Names
MT1G; MT1; MT1K
Reactivity
Human
Synonyms
Metallothionein-1G (MT1G); N/A; Human Metallothionein-1G (MT1G) ELISA Kit; MT1G; MGC12386; MT1; MT1K; metallothionein 1K; MT1G elisa kit
Reactivity
Human
Specificity
Human Metallothionein-1G (MT1G) ELISA Kit has high sensitivity and excellent specificity for detection of Human MT1G. No significant cross-reactivity or interference
Sequence Length
61
Assay Type
Sandwich
Samples
Human serum, cell culture supernates and other biological fluids
Detection Range
100 ng/L - 1600 ng/L
Sensitivity
< 10 ng/L
Intra-assay Precision
Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?9%.
Inter-assay Precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?11%.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for MT1G elisa kit
Background: In 1957, American scientist Margoshoes isolated the metal protein of cadmium from animal organs while studying the role of metal biology. Because it is a low molecular weight, high sulfhydryl content and can bind a large amount of heavy metal ions, it is called metallothionein (referred to as MT). The MT molecule is oval in shape, divided into two domains, with a molecular weight of 6,000-7,000 Daltons, a diameter of 30-50Å, and contains 61 amino acids, of which 20 amino acids are cysteine, and a few have 21, so that each MT The molecule can bind 7 to 12 metal ions. Humans, animals, plants and microorganisms all contain MT, and their physical and chemical properties are basically the same, with special light absorption. The MT conformation is stronger and has strong heat resistance.
Principle of the Assay: Human Metallothionein-1G (MT1G) ELISA Kit employs a two-site sandwich ELISA to quantitate MT1G in samples. An antibody specific for MT1G has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MT1G present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human MT1G detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of MT1G bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: Human Metallothionein-1G (MT1G) ELISA Kit employs a two-site sandwich ELISA to quantitate MT1G in samples. An antibody specific for MT1G has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MT1G present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human MT1G detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of MT1G bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
metallothionein-1G isoform 1
NCBI Official Synonym Full Names
metallothionein 1G
NCBI Official Symbol
MT1G
NCBI Official Synonym Symbols
MT1; MT1K
NCBI Protein Information
metallothionein-1G
UniProt Protein Name
Metallothionein-1G
UniProt Gene Name
MT1G
UniProt Synonym Gene Names
MT1K; MT1M; MT-1G; MT-1K; MT-IG
UniProt Entry Name
MT1G_HUMAN
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Product Notes
The Human MT1G mt1g (Catalog #AAA331673) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331673 ELISA Kit recognizes Human MT1G. It is sometimes possible for the material contained within the vial of "Metallothionein-1G (MT1G), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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