Human Meteorin Like Protein (METRNL) ELISA Kit | METRNL elisa kit
Human Meteorin Like Protein (METRNL) ELISA Kit
Reactivity
Human
Synonyms
Meteorin Like Protein (METRNL); N/A; Human Meteorin Like Protein (METRNL) ELISA Kit; Subfatin; Glial Cell Differentiation Regulator Like; METRNL elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of METRNL. No significant cross-reactivity or interference between METRNL and analogues was observed.
Sequence Length
311
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Detection Range
1.56-100ng/mL
Sensitivity
0.63ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level METRNL were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level METRNL were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Related Product Information for METRNL elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of METRNL in human serum, plasma, tissue homogenates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to METRNL. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to METRNL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain METRNL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of METRNL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to METRNL. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to METRNL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain METRNL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of METRNL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for METRNL elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,738 Da
NCBI Official Full Name
meteorin-like protein
NCBI Official Synonym Full Names
meteorin, glial cell differentiation regulator-like
NCBI Official Symbol
METRNL
NCBI Protein Information
meteorin-like protein
UniProt Protein Name
Meteorin-like protein
UniProt Gene Name
METRNL
UniProt Entry Name
METRL_HUMAN