Standard Curve (Sample)
Mouse Meteorin-like/METRNL ELISA Kit | METRNL elisa kit
Mouse Meteorin-like/METRNL PicoKine ELISA Kit
Gene Names
Metrnl; BC019776; 9430048M07Rik
Reactivity
Mouse
Synonyms
Meteorin-like/METRNL; N/A; Mouse Meteorin-like/METRNL PicoKine ELISA Kit; Meteorin-like protein; Metrnl; Subfatin; Q8VE43; meteorin, glial cell differentiation regulator-like; METRNL elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Metrnl
Sequence Length
311
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
62.5 pg/ml - 4,000 pg/ml
Sensitivity
<12 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for METRNL elisa kit
Background: METRNL (Meteorin-like) is a cytoplasmic 30 kDa (predicted) member of the meteorin family of proteins. It is expressed by neurons, and appears to function as a primer molecule that sets the stage for either cell differentiation or neurite outgrowth when it acts with Dclk1 and SerpinB1. In mice, exogenously elevated circulating Metrnl increased whole-body energy expenditure and improved glucose tolerance following diet-induced obesity. It also induced expression of antiinflammatory genes, leading to alternative activation of macrophages in adipose tissue and to browning of white fat depots. Metrnl did not appear to activate thermogenic genes directly, but it stimulated eosinophils and macrophages to enter adipose tissue and take their specific thermogenic actions. Blockade of cytokine signaling eliminated Metrnl-induced changes in gene expression required for alternative macrophage activation and blunted expression of beige fat thermogenic and beta-oxidation genes. It is concluded that METRNL is a hormone that influences energy expenditure and thermogenic programs in adipose tissue.
Principle of the Assay: The Picokine™ Mouse Metrnl Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Metrnl with a 96-well strip plate that is pre-coated with antibody specific for Metrnl. The detection antibody is a biotinylated antibody specific for Metrnl. The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Metrnl with immunogen: Expression system for standard: NS0; Immunogen sequence: Q46-E311. The kit is analytically validated with ready-to-use reagents. To measure Mouse Metrnl, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Metrnl in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Metrnl in the sample.
Principle of the Assay: The Picokine™ Mouse Metrnl Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Metrnl with a 96-well strip plate that is pre-coated with antibody specific for Metrnl. The detection antibody is a biotinylated antibody specific for Metrnl. The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Metrnl with immunogen: Expression system for standard: NS0; Immunogen sequence: Q46-E311. The kit is analytically validated with ready-to-use reagents. To measure Mouse Metrnl, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Metrnl in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Metrnl in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,721 Da
NCBI Official Full Name
meteorin-like protein
NCBI Official Synonym Full Names
meteorin, glial cell differentiation regulator-like
NCBI Official Symbol
Metrnl
NCBI Official Synonym Symbols
BC019776; 9430048M07Rik
NCBI Protein Information
meteorin-like protein
UniProt Protein Name
Meteorin-like protein
UniProt Gene Name
Metrnl
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Product Notes
The Mouse METRNL metrnl (Catalog #AAA46779) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA46779 ELISA Kit recognizes Mouse METRNL. It is sometimes possible for the material contained within the vial of "Meteorin-like/METRNL, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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