Standard Curve (Sample)
(Fig.1. Human Methionine aminopeptidase 2 (METAP2) Standard Curve.)
Human Methionine Aminopeptidase 2 (METAP2) ELISA Kit | METAP2 elisa kit
Human Methionine Aminopeptidase 2 (METAP2) ELISA Kit
Gene Names
METAP2; MAP2; MNPEP; p67eIF2
Reactivity
Human
Synonyms
Methionine Aminopeptidase 2 (METAP2); N/A; Human Methionine Aminopeptidase 2 (METAP2) ELISA Kit; METAP2; MAP2; MNPEP; p67; p67eIF2; eIF-2-associated p67 homolog; initiation factor 2-associated 67 kDa glycoprotein; peptidase M 2; METAP2 elisa kit
Reactivity
Human
Specificity
Human Methionine aminopeptidase 2 (METAP2) ELISA Kit has high sensitivity and excellent specificity for detection of Human METAP2. No significant cross-reactivity or interference between Human METAP2 and analogues was observed.
Sequence Length
478
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Methionine aminopeptidase 2 (METAP2) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Methionine aminopeptidase 2 (METAP2) Standard Curve.)
Related Product Information for METAP2 elisa kit
Description: This Human Methionine aminopeptidase 2 (METAP2) ELISA Kit employs a two-site sandwich ELISA to quantitate METAP2 in samples. An antibody specific for METAP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any METAP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for METAP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of METAP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Methionine aminopeptidase 2 encoded by METAP2 is a member of the methionyl aminopeptidase family. Methionine aminopeptidase 2 functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent proteins. Increased expression of METAP2 is associated with various forms of cancer, and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. Inhibitors of this gene have also been shown to be effective for the treatment of obesity. A pseudogene of METAP2 is located on chromosome 2. Several transcript variants encoding different isoforms have been found for METAP2.
Background: Methionine aminopeptidase 2 encoded by METAP2 is a member of the methionyl aminopeptidase family. Methionine aminopeptidase 2 functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent proteins. Increased expression of METAP2 is associated with various forms of cancer, and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. Inhibitors of this gene have also been shown to be effective for the treatment of obesity. A pseudogene of METAP2 is located on chromosome 2. Several transcript variants encoding different isoforms have been found for METAP2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
methionine aminopeptidase 2 isoform 1
NCBI Official Synonym Full Names
methionyl aminopeptidase 2
NCBI Official Symbol
METAP2
NCBI Official Synonym Symbols
MAP2; MNPEP; p67eIF2
NCBI Protein Information
methionine aminopeptidase 2
UniProt Protein Name
Methionine aminopeptidase 2
UniProt Gene Name
METAP2
UniProt Synonym Gene Names
MNPEP; P67EIF2; MAP 2
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Product Notes
The Human METAP2 metap2 (Catalog #AAA331793) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331793 ELISA Kit recognizes Human METAP2. It is sometimes possible for the material contained within the vial of "Methionine Aminopeptidase 2 (METAP2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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