Rabbit anti-Human methionyl aminopeptidase 2/METAP2 Polyclonal Antibody | anti-METAP2 antibody

Anti-methionyl aminopeptidase 2/METAP2 Antibody, Rabbit Polyclonal

Cat. Num. AAA254758

• Gene Names: METAP2; MAP2; MNPEP; p67eIF2
• Reactivity: Human
• Applications: Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: methionyl aminopeptidase 2/METAP2, Antibody; Anti-methionyl aminopeptidase 2/METAP2 Antibody, Rabbit Polyclonal; MAP2/METAP2 Antibody, Rabbit PAb, Antigen Affinity Purified; methionyl aminopeptidase 2; Anti-MAP2 Antibody; Anti-MNPEP Antibody; Anti-p67 Antibody; Anti-p67eIF2 Antibody; anti-METAP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human methionyl aminopeptidase 2/METAP2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-METAP2 antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Immunogen: Recombinant Human MAP2/METAP2 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human MAP2/METAP2 (rh MAP2/METAP2). MAP2/METAP2 specific IgG was purified by Human MAP2/METAP2 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Anti-METAP2 rabbit polyclonal antibody at 1:500 dilutionLane A: METAP2 konckout Hela Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 10 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:52 kDaObserved band size:72 kDa)

IP (Immunoprecipitation)

(METAP2 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate4 uL anti-METAP2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-METAP2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 52 kDaObserved band size: 72 kDa)

IHC (Immunohiostchemistry)

(Immunochemical staining of human METAP2 in human small intestine with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)

WB (Western Blot)

(Anti-METAP2 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: PC12 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:52 kDaObserved band size:72 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-METAP2 antibody

METAP2 (Methionine aminopeptidase 2), also known as MAP2 is a protein that belongs to the peptidase M24A family. MAP2 binds 2 cobalt or manganese ions and contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. It is found in all organisms and is especially important because of its critical role in tissue repair and protein degradation. The catalytic activity of human MAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. MAP2 protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. It also plays a critical role in the regulation of protein synthesis.

References

Bennett, et al. (1997) EPR Studies on the Mono-and Dicobalt (II)-Substituted Forms of the Aminopeptidase from Aeromonas proteolytica. Insight into the Catalytic Mechanism of Dinuclear Hydrolases. J Am Chem Soc. 119:1923-33.Johansson, et al. (2008) Dicobalt II-II, II-III, and III-III Complexes as Spectroscopic Models for Dicobalt Enzyme Active Sites. Inorg Chem. 47:5079-92.Bradshaw, et al. (2002) Aminopeptidases and angiogenesis. Essays Biochem. 38: 5-78.

NCBI and Uniprot Product Information

• NCBI GI #: 5803092
• NCBI GeneID: 10988
• NCBI Accession #: NP_006829.1
• NCBI GenBank Nucleotide #: NM_006838.3
• UniProt Accession #: P50579; Q8NB11; B2RDI8; B4DUX5; G3XA91
• Molecular Weight: 50,510 Da
• NCBI Official Full Name: methionine aminopeptidase 2 isoform 1
• NCBI Official Synonym Full Names: methionyl aminopeptidase 2
• NCBI Official Symbol: METAP2
• NCBI Official Synonym Symbols: MAP2; MNPEP; p67eIF2
• NCBI Protein Information: methionine aminopeptidase 2
• UniProt Protein Name: Methionine aminopeptidase 2
• UniProt Gene Name: METAP2
• UniProt Synonym Gene Names: MNPEP; P67EIF2; MAP 2