Human MICB ELISA Kit | MICB elisa kit

Human MICB ELISA Kit

Cat. Num. AAA105650

• Gene Names: MICB; PERB11.2
• Reactivity: Human
• Applications: ELISA
• Synonyms: MICB; N/A; Human MICB ELISA Kit; PERB11.2; MHC class I polypeptide-related sequence B; MIC-B; MICB elisa kit

• Reactivity: Human
• Specificity: The Human MICB ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human MICB proteins within the range of156 pg/ml - 10000 pg/ml.
• Sequence Length: 351
• Samples: Culture Supernatants, Serum, Plasma
• Sensitivity: < 80 pg/ml

• Applicable Applications for MICB elisa kit: ELISA (Sandwich ELISA)
• Detection Method: Colorimetric
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Standard Curve (Sample)

Related Product Information for MICB elisa kit

Background: MHC class I polypeptide-related sequence B, also called MICB is a protein that in humans is encoded by the MICB gene. The MICB gene is mapped to 6p21.33. This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. It seems to have no role in antigen presentation. This gene acts as a stress-induced self-antigen that is recognized by gamma delta T cells. It is the ligand for the KLRK1/NKG2D receptor and binds to KLRK1 leads to cell lysis.

Principle of the Assay: The AAA Biotech' Human MICB ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MICB in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human MICB coated on a 96-well plate. Standards and samples are pipetted into the wells and MICB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MICB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MICB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 574584674
• NCBI GeneID: 4277
• NCBI Accession #: NP_001276089.1
• NCBI GenBank Nucleotide #: NM_001289160.1
• UniProt Accession #: Q29980; O14499; O14500; O19798; O19799; O19800; O19801; O19802; A2AC57; A6NP85; B0UZ10; B2RAK2
• Molecular Weight: 4,287 Da
• NCBI Official Full Name: MHC class I polypeptide-related sequence B isoform 2
• NCBI Official Synonym Full Names: MHC class I polypeptide-related sequence B
• NCBI Official Symbol: MICB
• NCBI Official Synonym Symbols: PERB11.2
• NCBI Protein Information: MHC class I polypeptide-related sequence B; MICB; MHC class I mic-B antigen; stress inducible class I homolog; MHC class I chain-related protein B; MHC class I-like molecule PERB11.2-IMX
• UniProt Protein Name: MHC class I polypeptide-related sequence B
• UniProt Gene Name: MICB
• UniProt Synonym Gene Names: MIC-B
• UniProt Entry Name: MICB_HUMAN