Standard Curve (Sample)
(Fig.1. Human Microtubule-associated serine/threonine-protein kinase 3 (MAST3) Standard Curve.)
Human Microtubule-Associated Serine/Threonine-Protein Kinase 3 (MAST3) ELISA Kit | MAST3 elisa kit
Human Microtubule-Associated Serine/Threonine-Protein Kinase 3 (MAST3) ELISA Kit
Reactivity
Human
Synonyms
Microtubule-Associated Serine/Threonine-Protein Kinase 3 (MAST3); N/A; Human Microtubule-Associated Serine/Threonine-Protein Kinase 3 (MAST3) ELISA Kit; MAST3; KIAA0561; MAST3 elisa kit
Reactivity
Human
Specificity
Human Microtubule-associated serine/threonine-protein kinase 3 (MAST3) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAST3. No significant cross-reactivity or interference between Human MAST3 and analogues was observed.
Sequence Length
1309
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Microtubule-associated serine/threonine-protein kinase 3 (MAST3) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Microtubule-associated serine/threonine-protein kinase 3 (MAST3) Standard Curve.)
Related Product Information for MAST3 elisa kit
Description: This Human Microtubule-associated serine/threonine-protein kinase 3 (MAST3) ELISA Kit employs a two-site sandwich ELISA to quantitate MAST3 in samples. An antibody specific for MAST3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAST3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAST3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAST3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: By sequencing clones obtained from a size-fractionated brain cDNA library, Nagase et al. (1998) cloned MAST3, which they designated KIAA0561. The transcript contains repetitive elements in its 3-prime UTR, and the deduced 1,308-amino acid protein shares significant similarity with mouse Mast205 (MAST2) and human MAST4. RT-PCR detected variable MAST3 expression in all tissues examined, with highest levels in kidney, brain, and small intestine.Valiente et al. (2005) showed that the C-terminal tail of human PTEN bound to the PDZ domains of rat Magi2, Magi3, and Dlg (DLG1), mouse Sast (MAST1) and Mast205, and human MAST3. Interaction of PTEN with Magi2 increased PTEN protein stability, and interaction of PTEN with the MAST kinases facilitated phosphorylation of PTEN by these kinases.
Background: By sequencing clones obtained from a size-fractionated brain cDNA library, Nagase et al. (1998) cloned MAST3, which they designated KIAA0561. The transcript contains repetitive elements in its 3-prime UTR, and the deduced 1,308-amino acid protein shares significant similarity with mouse Mast205 (MAST2) and human MAST4. RT-PCR detected variable MAST3 expression in all tissues examined, with highest levels in kidney, brain, and small intestine.Valiente et al. (2005) showed that the C-terminal tail of human PTEN bound to the PDZ domains of rat Magi2, Magi3, and Dlg (DLG1), mouse Sast (MAST1) and Mast205, and human MAST3. Interaction of PTEN with Magi2 increased PTEN protein stability, and interaction of PTEN with the MAST kinases facilitated phosphorylation of PTEN by these kinases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
microtubule-associated serine/threonine-protein kinase 3
NCBI Official Synonym Full Names
microtubule associated serine/threonine kinase 3
NCBI Official Symbol
MAST3
NCBI Protein Information
microtubule-associated serine/threonine-protein kinase 3
UniProt Protein Name
Microtubule-associated serine/threonine-protein kinase 3
UniProt Gene Name
MAST3
UniProt Synonym Gene Names
KIAA0561
UniProt Entry Name
MAST3_HUMAN
Similar Products
Product Notes
The Human MAST3 mast3 (Catalog #AAA331866) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331866 ELISA Kit recognizes Human MAST3. It is sometimes possible for the material contained within the vial of "Microtubule-Associated Serine/Threonine-Protein Kinase 3 (MAST3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.