SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
MAST3 recombinant protein
MAST3, Active
Applications
Western Blot
Synonyms
MAST3; N/A; MAST3, Active; MAST3 recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, and 25% glycerol.
Applicable Applications for MAST3 recombinant protein
WB (Western Blot), Kinase Assay
Tag
GST
Species
Human
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Related Product Information for MAST3 recombinant protein
Recombinant human MAST3 (1-723a.a.) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number for MAST3 is NM_015016.
MAST3 belongs to the microtubule-associated serine/threonine-protein kinase family, which is characterized by containing a serine/threonine kinase domain and a PDZ domain, and is highly expressed in brain (1). MSAT3 suppresses the degradation and promotes phosphorylation of tumor suppressor PTEN via binding to specific PDZ domains (2). MSAT3 is known to modulate the immune functions in inflammatory bowel diseases via regulating NF-kB, cell adhesion and/or migration and production of pro-inflammatory cytokines (3).
MAST3 belongs to the microtubule-associated serine/threonine-protein kinase family, which is characterized by containing a serine/threonine kinase domain and a PDZ domain, and is highly expressed in brain (1). MSAT3 suppresses the degradation and promotes phosphorylation of tumor suppressor PTEN via binding to specific PDZ domains (2). MSAT3 is known to modulate the immune functions in inflammatory bowel diseases via regulating NF-kB, cell adhesion and/or migration and production of pro-inflammatory cytokines (3).
Product Categories/Family for MAST3 recombinant protein
References
1. Garlanda, P. et al. Expression of the MAST family of serine/threonine kinases. Brain Research. (1195) 12-19, 2008.
2. Valiente, M. et al. Binding of PTEN to Specific PDZ Domains Contributes to PTEN Protein Stability and Phosphorylation by Microtubule-associated Serine/Threonine Kinases. The Journal of Biological Chemistry. (280) 28936-28943, 2005.
3. Labbé, C. et al. Genome-wide Expression Profiling Implicates a MAST3-Regulated Gene Set in Colonic Mucosal Inflammation of Ulcerative Colitis Patients. Inflamm Bowel Dis. (6) 1072-1080, 2012.
2. Valiente, M. et al. Binding of PTEN to Specific PDZ Domains Contributes to PTEN Protein Stability and Phosphorylation by Microtubule-associated Serine/Threonine Kinases. The Journal of Biological Chemistry. (280) 28936-28943, 2005.
3. Labbé, C. et al. Genome-wide Expression Profiling Implicates a MAST3-Regulated Gene Set in Colonic Mucosal Inflammation of Ulcerative Colitis Patients. Inflamm Bowel Dis. (6) 1072-1080, 2012.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The MAST3 (Catalog #AAA73736) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MAST3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Kinase Assay. Researchers should empirically determine the suitability of the MAST3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAST3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.