Human MT ELISA Kit | MT elisa kit
Human MT (Melatonin) ELISA Kit
Reactivity
Human
Synonyms
MT; N/A; Human MT (Melatonin) ELISA Kit; MT elisa kit
Reactivity
Human
Specificity
This kit recognizes natural and recombinant Human MT. No significant cross-reactivity or interference between Human MT and analogues was observed.
Sequence Length
60
Samples
Serum, Plasma, Biological Fluids
Assay Type
Competitive
Detection Range
15.625-1000pg/mL
Sensitivity
Min: 9.375pg/mL; Max: 1000pg/mL
Intended Uses
This ELISA kit applies to the in vitro quantitative determination of Human MT concentrations in serum, plasma and other biological fluids.
Preparation and Storage
Store at 4 degree C.
Standard Curve (Sample)
Related Product Information for MT elisa kit
Principle of the assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with MT. During the reaction, MT in the sample or standard competes with a fixed amount of MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to MT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MT in the samples is then determined by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
6,007 Da
NCBI Official Full Name
Metallothionein
NCBI Official Symbol
LOC100049397
NCBI Protein Information
metallothionein; MT
UniProt Protein Name
Metallothionein
UniProt Gene Name
mt
UniProt Synonym Gene Names
MT
UniProt Entry Name
MT_ORYLA
