Standard Curve (Sample)
Human Myosin-Ic (MYO1C) ELISA Kit | MYO1C elisa kit
Human Myosin-Ic (MYO1C) ELISA Kit
Gene Names
MYO1C; NMI; MMIb; myr2; MMI-beta
Reactivity
Human
Synonyms
Myosin-Ic (MYO1C); N/A; Human Myosin-Ic (MYO1C) ELISA Kit; MYO1C; FLJ23903; MMI-beta; MMIb; NMI; myr2; myosin-I beta; nuclear myosin I; MYO1C elisa kit
Reactivity
Human
Specificity
Human Myosin-Ic (MYO1C) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYO1C. No significant cross-reactivity or interference between Human MYO1C and analogues was observed.
Sequence Length
1063
Assay Type
Sandwich
Samples
Human serum, cell culture supernates and other biological fluids
Detection Range
0.5 ng/ml - 8 ng/ml
Sensitivity
< 0.05ng/ml
Intra-assay Precision
Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?9%.
Inter-assay Precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?11%.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for MYO1C elisa kit
Background: MYO1C encodes a member of the unconventional myosin protein family, which are actin-based molecular motors. Myosin-Ic is found in the cytoplasm, and one isoform with a unique N-terminus is also found in the nucleus. The nuclear isoform associates with RNA polymerase I and II and functions in transcription initiation. The mouse ortholog of Myosin-Ic also functions in intracellular vesicle transport to the plasma membrane. Multiple transcript variants encoding different isoforms have been found for MYO1C. The related gene myosin IE has been referred to as myosin IC in the literature, but it is a distinct locus on chromosome 19.
Principle of the Assay: Human Myosin-Ic (MYO1C) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO1C in samples. An antibody specific for MYO1C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MYO1C present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human MYO1C detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of MYO1C bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: Human Myosin-Ic (MYO1C) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO1C in samples. An antibody specific for MYO1C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MYO1C present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human MYO1C detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of MYO1C bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Unconventional myosin-Ic
NCBI Official Synonym Full Names
myosin IC
NCBI Official Symbol
MYO1C
NCBI Official Synonym Symbols
NMI; MMIb; myr2; MMI-beta
NCBI Protein Information
unconventional myosin-Ic
UniProt Protein Name
Unconventional myosin-Ic
UniProt Gene Name
MYO1C
UniProt Synonym Gene Names
MMI-beta; MMIb
UniProt Entry Name
MYO1C_HUMAN
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Product Notes
The Human MYO1C myo1c (Catalog #AAA331586) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331586 ELISA Kit recognizes Human MYO1C. It is sometimes possible for the material contained within the vial of "Myosin-Ic (MYO1C), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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