Standard Curve (Sample)
(Fig.1. Human Myristoylated alanine-rich C-kinase substrate (MARCKS) Standard Curve.)
Human Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) ELISA Kit | MARCKS elisa kit
Human Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) ELISA Kit
Gene Names
MARCKS; MACS; 80K-L; PKCSL; PRKCSL
Reactivity
Human
Synonyms
Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS); N/A; Human Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) ELISA Kit; MARCKS; 80K-L; FLJ14368; FLJ90045; MACS; PKCSL; PRKCSL; myristoylated alanine-rich protein kinase C substrate (MARCKS; 80K-L); phosphomyristin; MARCKS elisa kit
Reactivity
Human
Specificity
Human Myristoylated alanine-rich C-kinase substrate (MARCKS) ELISA Kit has high sensitivity and excellent specificity for detection of Human MARCKS. No significant cross-reactivity or interference between Human MARCKS and analogues was observed.
Sequence Length
332
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Myristoylated alanine-rich C-kinase substrate (MARCKS) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Myristoylated alanine-rich C-kinase substrate (MARCKS) Standard Curve.)
Related Product Information for MARCKS elisa kit
Description: This Human Myristoylated alanine-rich C-kinase substrate (MARCKS) ELISA Kit employs a two-site sandwich ELISA to quantitate MARCKS in samples. An antibody specific for MARCKS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MARCKS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MARCKS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MARCKS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MARCKS is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. MARCKS is a filamentous actin crosslinking protein, with activity that is inhibited by PKC-mediated phosphorylation and by binding to calcium-calmodulin. MARCKS may be a regulated crossbridge between actin and the plasma membrane, and modulation of the actin crosslinking activity of the MARCKS protein by calmodulin and phosphorylation represents a potential convergence of the calcium-calmodulin and protein kinase C signal transduction pathways in the regulation of the actin cytoskeleton.
Background: MARCKS is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. MARCKS is a filamentous actin crosslinking protein, with activity that is inhibited by PKC-mediated phosphorylation and by binding to calcium-calmodulin. MARCKS may be a regulated crossbridge between actin and the plasma membrane, and modulation of the actin crosslinking activity of the MARCKS protein by calmodulin and phosphorylation represents a potential convergence of the calcium-calmodulin and protein kinase C signal transduction pathways in the regulation of the actin cytoskeleton.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
myristoylated alanine-rich C-kinase substrate
NCBI Official Synonym Full Names
myristoylated alanine rich protein kinase C substrate
NCBI Official Symbol
MARCKS
NCBI Official Synonym Symbols
MACS; 80K-L; PKCSL; PRKCSL
NCBI Protein Information
myristoylated alanine-rich C-kinase substrate
UniProt Protein Name
Myristoylated alanine-rich C-kinase substrate
UniProt Gene Name
MARCKS
UniProt Synonym Gene Names
MACS; PRKCSL; MARCKS; 80K-L protein; PKCSL
UniProt Entry Name
MARCS_HUMAN
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Product Notes
The Human MARCKS marcks (Catalog #AAA331871) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331871 ELISA Kit recognizes Human MARCKS. It is sometimes possible for the material contained within the vial of "Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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