Chicken MARCKS (Myristoylated Alanine Rich C Kinase Substrate) Polyclonal Antibody | anti-MARCKS antibody

MARCKS (Myristoylated Alanine Rich C Kinase Substrate)

Cat. Num. AAA76713

• Gene Names: MARCKS; MACS; 80K-L; PKCSL; PRKCSL
• Reactivity: Human, Primate
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, Immunocytochemistry
• Synonyms: MARCKS (Myristoylated Alanine Rich C Kinase Substrate), Antibody; MARCKS (Myristoylated Alanine Rich C Kinase Substrate); anti-MARCKS antibody

• Host: Chicken
• Reactivity: Human, Primate
• Clonality: Polyclonal
• Isotype: Chicken IgY
• Form/Format: Liquid. Supplied as an aliquot of IgYpreparation plus 5mM NaN3

• Applicable Applications for anti-MARCKS antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry)
• Immunogen: Full length recombinant human MARCKS expressed in and purified from E coli.
• Preparation and Storage: The antibody can be stored at 2 degree - 8 degree C for 12 months without detectable loss of activity. Avoidrepeated freeze-thaw cycles.

WB (Western Blot)

(Western blot analysis of different cell lines lysates using chicken pAB to MARCKS, AAA76713, dilution 1:1,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, and [5] COS1 cells. Strong band at 80kDa corresponds to MARCKS protein. The same blot was simultaneously probed with mouse mAb to GAPDH in red, which reveals a single band at 37kDa in all preparations.)

IF (Immunofluorescence)

(Immunofluorescent analysis of HeLa cells stained with chicken pAb to MARCKS, AAA76713, dilution 1:5,000 in red, and costained with mouse mAb to beta-tubulin in green. The blue is Hoechst staining of nuclear DNA. The MARCKS antibody binds to MARCKS protein expressed in the plasma membrane and cytoplasm, while the beta-tubulin antibody stains cytoplasmic microtubules.)

Related Product Information for anti-MARCKS antibody

Myristolyated alanine rich C-kinase substrate, hence MARCKS, was originally discovered by as a major substrate for protein kinase C in the brain and other tissues. MARCKS interacts with actin and calcium in a manner regulated by protein kinase C. It is a major protein of the brain concentrated in the synapses of neurons and is membrane localized due to the N-terminal lipid myristoyl group. Antibody to MARCKS can therefore be used as a marker of synaptic regions. Genetic knock out of MARCKS in transgenic mice is perinatal lethal and associated with aberrant brain development suggesting a fundamental importance in the CNS.

This antibody was made against recombinant full length human MARCKS expressed in and purified from E coli. The antibody works well on human cells and tissues but is not recommended for work on rodent material.

NCBI and Uniprot Product Information

• NCBI GI #: 153070260
• NCBI GeneID: 4082
• NCBI Accession #: NP_002347.5
• NCBI GenBank Nucleotide #: NM_002356.5
• UniProt Accession #: P29966; Q2LA83; Q5TDB7; E1P560
• Molecular Weight: 31,555 Da
• NCBI Official Full Name: myristoylated alanine-rich C-kinase substrate
• NCBI Official Synonym Full Names: myristoylated alanine-rich protein kinase C substrate
• NCBI Official Symbol: MARCKS
• NCBI Official Synonym Symbols: MACS; 80K-L; PKCSL; PRKCSL
• NCBI Protein Information: myristoylated alanine-rich C-kinase substrate; phosphomyristin; protein kinase C substrate, 80 kDa protein, light chain; myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L)
• UniProt Protein Name: Myristoylated alanine-rich C-kinase substrate
• UniProt Gene Name: MARCKS
• UniProt Synonym Gene Names: MACS; PRKCSL; MARCKS; 80K-L protein; PKCSL
• UniProt Entry Name: MARCS_HUMAN